POU3F2 抗体 (Internal Region)
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Quick Overview for POU3F2 抗体 (Internal Region) (ABIN498033)
抗原
See all POU3F2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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序列
- C-AQSLVQGDYG ALQSN
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特异性
- Recognizes POU3F2/BRN2/OCT7.
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交叉反应 (详细)
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Species reactivity (expected):Canine (Dog), Bovine and Porcine.
Species reactivity (tested):Human, Mouse and Rat. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence from the internal region (near N Terminus) of the protein sequence according to NP_005595.2 Genename: POU3F2
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应用备注
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Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.1-3 μg/mL. Approx 45 kDa band observed in Human Brain (Cerebellum) andin Mouse and Rat Brain lysates (calculated MW of 46.5 kDa according to HumanNP_005595.2 and 47.5 kDa according to Mouse NP_032925.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- POU3F2 (POU Class 3 Homeobox 2 (POU3F2))
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别名
- POU3F2
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背景
- POU3F2 is a protein belonging to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, which occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1) and Oct2 (POU2F2), and the pituitary protein Pit1 (PIT1). Class III POU genes are expressed predominantly in the CNS. It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression.Synonyms: BRN2, Brain-2, Brain-specific homeobox/POU domain protein 2, Brn-2, Nervous system-specific octamer-binding transcription factor N-Oct-3, OCT7, OTF-7, OTF7, Oct-7, Octamer-binding protein 7, Octamer-binding transcription factor 7, POU domain class 3 transcription factor 2
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基因ID
- 5454
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NCBI登录号
- NP_005595
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UniProt
- P20265
抗原
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