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POU3F2 抗体 (Internal Region)

The 山羊 多克隆 anti-POU3F2 antibody (ABIN498033) specifically detects POU3F2 in WB 和 EIA. The antibody is reactive with 人, 大鼠, 小鼠, Cow 和 Pig samples.
产品编号 ABIN498033
发货至: 中国
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Quick Overview for POU3F2 抗体 (Internal Region) (ABIN498033)

抗原

See all POU3F2 抗体
POU3F2 (POU Class 3 Homeobox 2 (POU3F2))

适用

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  • 6
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  • 1
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人, 大鼠, 小鼠, Cow, Pig

宿主

  • 30
  • 4
  • 1
山羊

克隆类型

  • 32
  • 3
多克隆

标记

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This POU3F2 antibody is un-conjugated

应用范围

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  • 7
  • 4
  • 2
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  • 2
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Western Blotting (WB), Enzyme Immunoassay (EIA)
  • 抗原表位

    • 7
    • 5
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    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
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    Internal Region

    序列

    C-AQSLVQGDYG ALQSN

    特异性

    Recognizes POU3F2/BRN2/OCT7.

    交叉反应 (详细)

    Species reactivity (expected):Canine (Dog), Bovine and Porcine.
    Species reactivity (tested):Human, Mouse and Rat.

    纯化方法

    Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence from the internal region (near N Terminus) of the protein sequence according to NP_005595.2 Genename: POU3F2
  • 应用备注

    Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.1-3 μg/mL. Approx 45 kDa band observed in Human Brain (Cerebellum) andin Mouse and Rat Brain lysates (calculated MW of 46.5 kDa according to HumanNP_005595.2 and 47.5 kDa according to Mouse NP_032925.1).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    POU3F2 (POU Class 3 Homeobox 2 (POU3F2))

    别名

    POU3F2

    背景

    POU3F2 is a protein belonging to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, which occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1) and Oct2 (POU2F2), and the pituitary protein Pit1 (PIT1). Class III POU genes are expressed predominantly in the CNS. It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression.Synonyms: BRN2, Brain-2, Brain-specific homeobox/POU domain protein 2, Brn-2, Nervous system-specific octamer-binding transcription factor N-Oct-3, OCT7, OTF-7, OTF7, Oct-7, Octamer-binding protein 7, Octamer-binding transcription factor 7, POU domain class 3 transcription factor 2

    基因ID

    5454

    NCBI登录号

    NP_005595

    UniProt

    P20265
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