PAX4 抗体 (Internal Region)
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北京 101111
Quick Overview for PAX4 抗体 (Internal Region) (ABIN498024)
抗原
See all PAX4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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序列
- C-GKLATATSLP EDTVR
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特异性
- Recognizes Paired Box 4 (PAX4).
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交叉反应 (详细)
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Species reactivity (expected):Rat, Canine (Dog).
Species reactivity (tested):Human, Mouse. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence from the Internal region of the protein sequence according to NP_006184.2. Genename: PAX4
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应用备注
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Peptide ELISA: Limit Dilution: 1/64000. Western blot: 0.2-0.6 μg/mL. Approx 37 kDa band observed in Mouse Pancreas lysates andin lysates of cell lines A431, HeLa and K562 (calculated MW of 37.7 kDa according to HumanNP_006184.2 and 38.7 kDa according to Mouse NP_035168.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- PAX4 (Paired Box 4 (PAX4))
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别名
- PAX4
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背景
- PAX4 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The paired box gene 4 is involved in pancreatic islet development and mouse studies have demonstrated a role for this gene in differentiation of insulin-producing beta- cells.Synonyms: Paired box protein Pax-4, Pax-4
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分子量
- 37.1 kDa (Human NP_006184.2) and 38.0 kDa (Mouse NP_035168.1).
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基因ID
- 5078
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NCBI登录号
- NP_006184
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UniProt
- O43316
抗原
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