HNRNPF 抗体 (N-Term)
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北京 101111
Quick Overview for HNRNPF 抗体 (N-Term) (ABIN497944)
抗原
See all HNRNPF 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-164, N-Term
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特异性
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Recognizes hnRNP-F/HNRNPF (1-174).
Cellular Localization: Nucleus/nucleoplasm -
交叉反应 (详细)
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Species reactivity (expected):Mouse (98 %), Rat (83 %), Bovine (97 %).
Species reactivity (tested):Human. -
纯化方法
- Antigen-Affinity Chromatography.
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免疫原
- Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 164 of hnRNP-F
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应用备注
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Western blotting: 1/500-1/3000. Immunofluorescence: 1/100-1/200. Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 1/100-1/250. Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.72 mg/mL
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缓冲液
- 0.1 M Tris, 0.1 M Glycine, 20 % Glycerol ( pH 7) containing 0.01 % Thimerosal as preservative.
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody undiluted (in aliquots) at -20 °C.
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- HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))
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别名
- HnRNP-F / HNRNPF
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背景
- HnRNP-F belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.Synonyms: HNRPF, Heterogeneous nuclear ribonucleoprotein F, Nucleolin-like protein mcs94-1
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基因ID
- 3185, 9606
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UniProt
- P52597
抗原
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