Pyruvate Orthophosphate Dikinase (PPDK) (AA 72-947) 抗体
The 兔 多克隆 anti-Pyruvate Orthophosphate Dikinase (PPDK) antibody is suitable to detect Pyruvate Orthophosphate Dikinase (PPDK) in samples from Arabidopsis thaliana, Cyanthobasis fruticulosa, Oryza sativa, Petrosimonia nigdeensis, Salsola grandis, Salsola tragus 和 Zea mays. It has been validated for WB.
产品编号 ABIN4966289
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Quick Overview for Pyruvate Orthophosphate Dikinase (PPDK) (AA 72-947) 抗体 (ABIN4966289)
抗原
Pyruvate Orthophosphate Dikinase (PPDK)
适用
Arabidopsis thaliana, Cyanthobasis fruticulosa, Oryza sativa, Petrosimonia nigdeensis, Salsola grandis, Salsola tragus, Zea mays
宿主
兔
克隆类型
多克隆
应用范围
Western Blotting (WB)
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抗原表位
- AA 72-947
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原理
- Anti-PPDK | Pyruvate orthophosphate dikinase
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交叉反应 （详细）
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Arabidopsis thaliana,Cyanthobasis fruticulosa, Oryza sativa, Petrosimonia nigdeensis, Salsola grandis, Salsola tragus, Zea mays
Not reactive in: Cucumis sativus -
预测反应
- Hordeum vulagre,Kalanchoe fedtschenkoi
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纯化方法
- Immunogen affinity purified serum
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免疫原
- Purified recomibinant enzyme consisting of residues 72-947 of Zea mays
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应用备注
- 1 : 25 000 (WB)
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说明
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PPDK levels inr C3 plants like Arabidopsis thaliana and Hordeum vulgare are very low and PPDK protein is very dilute in most tissues of C3 plants. To perform detection in C3 plants leaf proteins needs to be concentrated before western blot, Chastain et al. (2002).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 200 μL of sterile water in 40 % glycerol to a final protein concentration of 100 ng/μl
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缓冲液
- Immunogen affinity purified serum in PBS pH 7.4.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- Pyruvate Orthophosphate Dikinase (PPDK)
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背景
- PPDK (Pyruvate, phosphate dikinase 1) is involved in formation of phosphoenolpyruvate and activated by light-induced dephosphorylation. Inhibited by dark-induced phosphorylation. PPDK is a low-abundance enzyme in C3 plants while it is a key enzyme of C4 photosynthesis.
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分子量
- 102 | 95 kDa
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基因ID
- 827226
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UniProt
- O23404
抗原
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