Phosphoglucomutase 1 抗体
Quick Overview for Phosphoglucomutase 1 抗体 (ABIN4966274)
抗原
See all Phosphoglucomutase 1 (PGM1) 抗体适用
宿主
克隆类型
标记
应用范围
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预测反应
- Arabidopsis thaliana, Glycine max, Triticum aestivum
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免疫原
- Recombinant phosphoglucomutase of Zea mays, UniProt: P93804
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anti-Phosphoglucomutase 1 (PGM1) (AA 1-562) antibody
PGM1 适用: 人 WB, ELISA, IF, IP, RNAi 宿主: 小鼠 Monoclonal 3B8-H4 unconjugated
anti-Phosphoglucomutase 1 (PGM1) (AA 1-235) antibodyPGM1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Phosphoglucomutase 1 (PGM1) (AA 469-501) antibodyPGM1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB53795 unconjugated
anti-Phosphoglucomutase 1 (PGM1) (AA 1-562) antibodyPGM1 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Phosphoglucomutase 1 (PGM1) (AA 251-282) antibodyPGM1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB54238 unconjugated
anti-Phosphoglucomutase 1 (PGM1) (AA 1-562) antibodyPGM1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Phosphoglucomutase 1 (PGM1) antibodyPGM1 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphoglucomutase 1 (PGM1) (AA 467-516) antibodyPGM1 适用: 人, 小鼠, 大鼠, 豚鼠, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Phosphoglucomutase 1 (PGM1) (C-Term) antibodyPGM1 适用: 人, 小鼠, 大鼠, 兔, 豚鼠, 斑马鱼, Bat, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Phosphoglucomutase 1 (PGM1) antibodyPGM1 适用: 人, 小鼠, 大鼠 WB, IF, IC 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1:1000 (WB)
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说明
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Immunoprecipitation was performed by using Dynabeads ® Protein A: briefly 100 μl suspension was washed with 200 μl TTBS (Tris Buffered saline, 50 mM Tris-HCl pH 7.6 and 165 mM NaCl with 0.1% Tween 80) using the magnetic stands for concentrating the magnetic beads. After wash the beads were preincubated with 20 μl primary antibodies in 180 μl TTBS at room temperature for 30 minutes (minimum 15 minues). A first wash was followed afterwards with 200 μl TTBS and hence a real incubation with 200 μl plant extract (supernatant 20,000 x g for 3 min.), 200μl of TTBS and further 50 μl YeastBuster reagent (Novagen) containing a mixture of detergents to break and solubilize the mitochondria membrane. This incubation at room temperature was allowed to be under mild shaking to allow the beads to be in suspension. Hence supernatant was aspirated away by the use of the magnetic stand and two further washesing steps with 200 μl TTBS were performed prior mixing with 100 μl SDS-Sample buffer.Antibody is recognizing recombinant PGM1, overexpressed in E.coli.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water.
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Phosphoglucomutase 1 (PGM1)
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别名
- Phosphoglucomutase, cytoplasmic 1 (PGM1)
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背景
- Phosphoglucomutase is an enzyme which participates in both the breakdown and synthesis of glucose.
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分子量
- 63 kDa
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UniProt
- P93804
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途径
- Cellular Glucan Metabolic Process
抗原
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