MDH2 抗体
Quick Overview for MDH2 抗体 (ABIN4966235)
抗原
See all MDH2 抗体适用
宿主
克隆类型
标记
应用范围
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预测反应
- Arabidopsis thaliana, Brachypodium distachyon, Citrus sinensis, Coffea canephora, Cucumis sativus, Glycine max, Gossypium raimondii , Hordeum vulgare, Jatropha curcas, Leersia perrieri, Morus notabilis, Oryza sativa, Phaseolus vulgaris , Populus trichocarpa, Prunus persica, Ricinus communis, Setaria italica, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Zostera marina, Vitis vinifera
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免疫原
- Recombinant MDH2 of Zea mays, UniProt: B4FZU8
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anti-Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) (AA 25-338) antibody
MDH2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) antibodyKO Validated MDH2 适用: 人 WB, IHC (p), ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) (Internal Region) antibodyVerified MDH2 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) (AA 221-232) antibodyVerified MDH2 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) (AA 134-246) antibodyMDH2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G12 unconjugated
anti-Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) (AA 86-115) antibodyMDH2 适用: 人, 小鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB20827 unconjugated
anti-Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) (C-Term) antibodyMDH2 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 犬, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) (AA 59-338) antibodyMDH2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) (AA 134-246) antibodyMDH2 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1G12 unconjugated
anti-Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) (AA 134-246) antibodyMDH2 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2A7 unconjugated
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应用备注
- 1:1000 (WB)
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说明
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Immunoprecipitation was performed by using Dynabeads ® Protein A: briefly 100 μl suspension was washed with 200 μl TTBS (Tris Buffered saline, 50 mM Tris-HCl pH 7.6 and 165 mM NaCl with 0.1% Tween 80) using the magnetic stands for concentrating the magnetic beads. After wash the beads were preincubated with 20 μl primary antibodies in 180 μl TTBS at room temperature for 30 minutes (minimum 15 minues). A first wash was followed afterwards with 200 μl TTBS and hence a real incubation with 200 μl plant extract (supernatant 20,000 x g for 3 min.), 200μl of TTBS and further 50 μl YeastBuster reagent (Novagen) containing a mixture of detergents to break and solubilize the mitochondria membrane. This incubation at room temperature was allowed to be under mild shaking to allow the beads to be in suspension. Hence supernatant was aspirated away by the use of the magnetic stand and two further washesing steps with 200 μl TTBS were performed prior mixing with 100 μl SDS-Sample buffer.
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限制
- 仅限研究用
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溶解方式
- For reconstitution add 50 μL of sterile water.
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))
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别名
- Malate dehydrogenase 2 (mitochondrial) (MDH2)
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背景
- Malate dehydrogenase (EC=1.1.1.37) is an enzyme that reversibly catalyzes the oxidation of malate to oxaloacetate. This reaction is part of many metabolic pathways, including the citric acid cycle. Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. The protein is highly expressed in: young panicles and immature seeds, while its levels in roots and leaves are low. Alternative name: PP37.
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分子量
- 35 | 35 kDa
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UniProt
- B4FZU8
抗原
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