CCA1 抗体
The 兔 多克隆 anti-CCA1 antibody is suitable to detect CCA1 in samples from Arabidopsis thaliana. It has been validated for Co-IP 和 WB.
产品编号 ABIN4966136
发货至:
中国
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Quick Overview for CCA1 抗体 (ABIN4966136)
抗原
CCA1
(Circadian Clock Associated 1 (CCA1))
适用
Arabidopsis thaliana
宿主
兔
克隆类型
多克隆
应用范围
Co-Immunoprecipitation ( Co-IP), Western Blotting (WB)
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原理
- Anti-CCA1 | Circadian clock associated 1
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交叉反应 （详细）
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Arabidopsis thaliana
Not reactive in: Oryza sativa, Solanum lycopersicum -
纯化方法
- Immunogen affinity purified serum
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免疫原
- KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CCA1 protein sequence
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应用备注
- 1: 500 (Co-IP), 1 : 500 (WB)
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说明
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Important note about protein extractionTranscription factors are best isolated by freezing nucelar extract in liquid nitrogen after adding the extraction buffer, followed by thawing directly in 100°C heating block not in RT. When the samples were heated for 10 min, spinning of the extract for 5 min is needed to remove the cell debris. From the liquid extract measurement can be done and the rest, frozen in liquid Nitrogen, in aliquots. Frozen samples will not be thawed again to 100°C, but on RT and loaded as soon as they are liquid. Such precautions are necessary to take to be able to detect a transcription factor using antibodies.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Immunogen affinity purified serum in PBS pH 7.4.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- CCA1 (Circadian Clock Associated 1 (CCA1))
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背景
- CCA1 (Circadian clocl associated 1) encodes a transcriptional repressor that performs overlapping functions with LHY in a regulatory feedback loop that is closely associated with the circadian oscillator of Arabidopsis. Binds to the evening element in the promoter of TOC1 and represses TOC1 transcription. CCA1 and LHY colocalize in the nucleus and form heterodimers in vivo. CCA1 and LHY function synergistically in regulating circadian rhythms of Arabidopsis.
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分子量
- 67 | 80 kDa
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基因ID
- 819296
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UniProt
- P92973
抗原
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