BRI1 抗体
This anti-BRI1 antibody is a 兔 多克隆 antibody detecting BRI1 in WB 和 IP. Suitable for Arabidopsis thaliana.
Quick Overview for BRI1 抗体 (ABIN4966120)
抗原
BRI1
(Protein Brassinosteroid Insensitive 1 (BRI1))
适用
Arabidopsis thaliana
宿主
兔
克隆类型
多克隆
标记
This BRI1 antibody is un-conjugated
应用范围
Western Blotting (WB), Immunoprecipitation (IP)
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交叉反应 （详细）
- Not reactive in: Hordeum vulgare
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预测反应
- Brassica rapa
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免疫原
- KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BRI1 protein, Uniprot: O22476, TAIR: AT4G39400
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应用备注
- 1: 5000 (WB)
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说明
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Antibody was tested on bri1-1 and bri1-5 mutants. Bri1-1 is a point mutation in the kinase domain that renders the protein non-functional and plants compensate for that by over-accumulating the non-functional receptor. Bri1-5 is a mutant in the extracellular domain and the bri1-5 protein is retained in the ER. The bri1-5 plants contain less protein than the wild type and show an intermediate brassinosteroid deficient phenotype. Also BRI1-5 migrates higher than wild type BRI1 in SDS-PAGE, because it carries ER-type high mannose N-glycans. For IP: 15 μl GFP-trap beads was used for 200 mg plant material to precipitate GFP-tagged protein followed by detection with Co-IPed BRI1 on Western with 1:5000 diluted anti-BRI1 antibody.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water.
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储存条件
- -20 °C
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储存方法
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- BRI1 (Protein Brassinosteroid Insensitive 1 (BRI1))
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别名
- Brassinosteroid insensitive 1 (BRI1)
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背景
- BRI1 (Protein BRASSINOSTEROID INSENSITIVE 1) is a receptor which binds brassinolide and has a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates. Involved in a signaling cascade including expression of light- and stress-regulated genes, promotion of cell elongation, normal leaf and chloroplast senescence, and flowering. Alternative names:BRI1, BRASSINOSTEROID INSENSITIVE 1, CBB2, CABBAGE 2, DWF2, DWARF 2, BIN1, BR INSENSITIVE 1, ATBRI1, Brassinosteroid LRR receptor kinase
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分子量
- above 130 kDa (due to N-glycosylation)
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UniProt
- O22476
抗原
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