AGO1 抗体 (N-Term) (HRP)
Quick Overview for AGO1 抗体 (N-Term) (HRP) (ABIN4966093)
抗原
See all AGO1 (EIF2C1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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交叉反应 (详细)
- Not reactive in: Chlamydomonas reinhardtii
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预测反应
- Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Vitis vinifera
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免疫原
- N-terminal peptide of Arabidopsis thaliana AGO1 UniProt: O04379, TAIR: At1g48410.
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应用备注
- 1: 1000 with enhanced ECL (WB)
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说明
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AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.
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限制
- 仅限研究用
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状态
- Liquid
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储存方法
- Store at +4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
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- AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))
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别名
- argonaute 1 (AGO1)
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背景
- AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).This antibody is directly conjugated to Horseradish Peroxidase (HRP).
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分子量
- 116.4 | 130 kDa
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UniProt
- O04379
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途径
- Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization
抗原
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