AGO1 抗体 (N-Term) (Biotin)
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Quick Overview for AGO1 抗体 (N-Term) (Biotin) (ABIN4966092)
抗原
See all AGO1 (EIF2C1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- Anti-AGO1 | Argonaute 1 (40 μg, Biotin conjugated)
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交叉反应 （详细）
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Arabidopsis thaliana
Not reactive in: Chlamydomonas reinhardtii -
预测反应
- Brassica pekinensis,Capsella rubella,Malus domestica,Pisum sativum,Ricinus communis,Vitis vinifera
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纯化方法
- Immunogen affinity purified serum
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免疫原
- KLH-conugated N-terminal peptide of Arabidopsis thaliana AGO1 UniProt: O04379
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anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) antibody
EIF2C1 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 1-180) antibodyEIF2C1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (Internal Region) antibodyEIF2C1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (N-Term) antibodyEIF2C1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (Internal Region) antibodyVerified EIF2C1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 251-400) antibodyEIF2C1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (N-Term) antibodyEIF2C1 适用: 人, 小鼠, 大鼠, 犬, 猴, 兔, 马, Cow, 豚鼠, Pig, 非洲爪蟾, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 27-56) antibodyEIF2C1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) antibodyEIF2C1 适用: 人 WB 宿主: 小鼠 Monoclonal 24GB8080 unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 376-409) antibodyEIF2C1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1 : 1000 (WB)
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说明
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AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Immunogen affinity purified serum in PBS pH 7.4, conjugated to biotin.
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注意事项
- A preservative may be added for long time storage
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储存条件
- 4 °C
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储存方法
- Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
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有效期
- 12-24 months
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- AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))
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别名
- argonaute 1 (AGO1)
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背景
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Background: AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).This antibody is directly conjugated to Biotin.
Additional information: Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U.TCA acetone total protein precipitation method
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分子量
- 116.4 | 130 kDa
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基因ID
- 841262
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UniProt
- O04379
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途径
- Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization
抗原
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