WT1 抗体 (AA 346-375)
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北京 101111
Quick Overview for WT1 抗体 (AA 346-375) (ABIN3029622)
抗原
See all WT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 346-375
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交叉反应 (详细)
- Expected species reactivity: Mouse, Rat, Pig, Xenopus
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纯化方法
- Antigen affinity purified
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免疫原
- A portion of amino acids 346-375 from the human protein was used as the immunogen for this WT1 antibody.
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亚型
- Ig Fraction
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应用备注
- Titration of the WT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot the WT1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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- WT1 (Wilms Tumor 1 (WT1))
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别名
- WT1 (Wilms Tumor Protein)
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背景
- Wilms Tumor protein is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq].
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UniProt
- P19544
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途径
- Tube Formation
抗原
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