Ubiquitin 抗体 (N-Term)
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Quick Overview for Ubiquitin 抗体 (N-Term) (ABIN3029416)
抗原
See all Ubiquitin (Ub) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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交叉反应 （详细）
- Expected species reactivity: Bovine, C. elegans, Chicken, Drosophila, Hamster, Pig, Primate, Rabbit, Xenopus
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纯化方法
- Antigen affinity purified
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免疫原
- This Ubiquitin antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 25-57amino acids from the N-terminal region of the human protein.
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亚型
- Ig Fraction
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anti-Ubiquitin (Ub) antibody
Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ub) (AA 1-229) antibodyUb 适用: 人, 大鼠, 小鼠, Cow, Pig, 犬, 马, 兔 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ub) (AA 1-229) antibodyUb 适用: 人, 大鼠 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal D2 unconjugated
anti-Ubiquitin (Ub) (acLys27) antibodyUb 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ub) antibodyUb 适用: 人, 大鼠, 小鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 5F1 unconjugated
anti-Ubiquitin (Ub) antibody (HRP)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal HRP
anti-Ubiquitin (Ub) antibody (FITC)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal FITC
anti-Ubiquitin (Ub) antibody (Biotin)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal Biotin
anti-Ubiquitin (Ub) antibody (PE)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal PE
anti-Ubiquitin (Ub) antibody (APC)Ub 适用: Cow WB, IHC, ICC, IF, IP 宿主: 兔 Polyclonal APC
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应用备注
- Titration of the Ubiquitin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- In 1X PBS pH 7.4 with 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot the Ubiquitin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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- Ubiquitin (Ub)
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别名
- Ubiquitin
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背景
- Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair, Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation, Lys-29-linked is involved in lysosomal degradation, Lys-33-linked is involved in kinase modification, Lys-48-linked is involved in protein degradation via the proteasome, Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
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UniProt
- P0CG47
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途径
- Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
抗原
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