HDAC4 抗体 (AA 1052-1084)
Quick Overview for HDAC4 抗体 (AA 1052-1084) (ABIN3031232)
抗原
See all HDAC4 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 1052-1084
-
纯化方法
- Purified
-
免疫原
- A portion of amino acids 1052-1084 from the human protein was used as the immunogen for this HDAC4 antibody.
-
亚型
- Ig Fraction
-
-
anti-Histone Deacetylase 4 (HDAC4) (AA 904-1076) antibody
HDAC4 适用: 小鼠 WB, IHC, IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 4 (HDAC4) (AA 551-648) antibodyHDAC4 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2B11 unconjugated
anti-Histone Deacetylase 4 (HDAC4) (AA 456-592) antibodyHDAC4 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 7E2E6 unconjugated
anti-Histone Deacetylase 4 (HDAC4) (AA 456-592) antibodyHDAC4 适用: 人 ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 5E4A5 unconjugated
anti-Histone Deacetylase 4 (HDAC4) (AA 451-650) antibodyHDAC4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 4 (HDAC4) (AA 1049-1077) antibodyHDAC4 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 4 (HDAC4) antibodyHDAC4 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 4 (HDAC4) (pSer632) antibodyHDAC4 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 4 (HDAC4) (AA 530-630) antibodyHDAC4 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone Deacetylase 4 (HDAC4) antibodyHDAC4 适用: 人, 小鼠, 大鼠 WB, IF (cc) 宿主: 兔 Monoclonal 6B10 unconjugated
-
-
应用备注
- Titration of the HDAC4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Aliquot the HDAC4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
-
-
- HDAC4 (Histone Deacetylase 4 (HDAC4))
-
别名
- HDAC4
-
背景
- DNA is wrapped around histone proteins to form nucleosomes and chromatin fiber, a higher-order structure. Chromatin can become alternatively revealed to or concealed from transcription factors. Acetylation of lysine residues induces conformational changes in core histones by destabilizing nucleosomes and allowing transcription factors access to recognition elements in DNA. Deacetylation of histones by histone deacetylases (HDACs) reseals the chromosomal package, leading to a repression of transcription. HDAC4 does not bind DNA directly, but rather through MEF2C and MEF2D. Binding of the N terminus of HDAC4 to MEF2C represses MEF2C transcription activity. The catalytic domain of HDAC4 interacts with HDAC3 via the transcriptional corepressor NCOR2. Experimental conditions leading to the suppression of HDAC4 binding to NCOR2 and to HDAC3 result in loss of enzymatic activity associated with HDAC4, indicating regulation of transcription by bridging the enzymatically active NCOR2-HDAC3 complex and select transcription factors. HDAC4 and MITR contain calmodulin-binding domains that overlap with their MEF2 binding domains. Binding of calmodulin to HDAC4 leads to its dissociation from MEF2, relieving MEF2 from the transcriptional repression by HDAC4. Together, HDAC4, MITR, and CABIN1 constitute a family of calcium-sensitive transcriptional repressors of MEF2. In murine studies, HDAC4, which is expressed in prehypertrophic chondrocytes, interacts with and inhibits the activity of Runx2 in mice, a transcription factor necessary for chondrocyte hypertrophy, establishing HDAC4 as a central regulator of chondrocyte hypertrophy and skeletogenesis.
-
UniProt
- P56524
-
途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process
抗原
-
