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HIST1H4A 抗体 (meLys20)

This 兔多克隆 antibody specifically detects HIST1H4A in ELISA, WB, IHC, FACS 和 DB. It exhibits reactivity toward 人.

IHC

Dot blot analysis of H4 antibody (Methyl 2/methylation-specific). (HIST1H4A 抗体 (meLys20))

H4 antibody flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control (top histogram). (HIST1H4A 抗体 (meLys20))

FACS

Western blot analysis of lysate from K562 cell line using H4 antibody diluted at 1:1000 for each lane. (HIST1H4A 抗体 (meLys20))

FACS

7 images

产品编号 ABIN3031210

发货至: 中国

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Quick Overview for HIST1H4A 抗体 (meLys20) (ABIN3031210)

抗原

See all HIST1H4A 抗体

HIST1H4A (Histone Cluster 1, H4a (HIST1H4A))

适用

人

宿主

兔

克隆类型

多克隆

标记

This HIST1H4A antibody is un-conjugated

应用范围

ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Dot Blot (DB)

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meLys20

纯化方法

Antigen affinity purified

免疫原

Amino acids surrounding the K20 methylation site of the human protein were used as the immunogen for this Histone H4 antibody.

亚型

Ig Fraction
anti-Histone Cluster 1, H4a (HIST1H4A) (H4K16ac) antibody
HIST1H4A 适用: 人 ELISA, WB, IF, ChIP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (H4K31ac) antibody
HIST1H4A 适用: 人 ELISA, WB, IF, ChIP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (H4K12ac) antibody
HIST1H4A 适用: 人 ELISA, IF, ChIP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (H4K8ac) antibody
HIST1H4A 适用: 人 ELISA, IF, ChIP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (H4K79ac) antibody
HIST1H4A 适用: 人 ELISA, IF, ChIP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (2meLys20), (AA 1-30) antibody
HIST1H4A 适用: 人 WB, FACS, DB, IHC (p) 宿主: 兔 Polyclonal RB17115 unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (AA 1-12) antibody
HIST1H4A 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (AA 10-22) antibody
HIST1H4A 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (AA 74-85) antibody
HIST1H4A 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (AA 2-50) antibody
HIST1H4A 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

Titration of the H4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Dot blot: 1:500,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

限制

仅限研究用
状态

Liquid

缓冲液

In 1X PBS pH 7.4 with 0.09 % sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Aliquot the H4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
抗原

HIST1H4A (Histone Cluster 1, H4a (HIST1H4A))

别名

H4

背景

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones.

UniProt

P62805