GST-Tag 抗体
Quick Overview for GST-Tag 抗体 (ABIN3029803)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Purified
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免疫原
- Purified recombinant fusion protein was used to produced this monoclonal GST tag antibody.
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亚型
- IgG1
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anti-GST-Tag (AA 1-218) antibody
适用: Various Species WB, IP, DB 宿主: 小鼠 Monoclonal C9 unconjugated
anti-GST-Tag (AA 1-218) antibody适用: Various Species WB, IP, DB 宿主: 兔 Polyclonal unconjugated
anti-GST-Tag antibodyWB, IP 宿主: 小鼠 Monoclonal S-tag-05 unconjugated
anti-GST-Tag antibodyWB 宿主: 小鼠 Monoclonal 9AT106 unconjugated
anti-GST-Tag antibodyWB 宿主: 兔 Polyclonal RB2165 unconjugated
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应用备注
- Titration of the GST Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000-2000
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- In 1X PBS pH 7.4 with 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot the GST Tag antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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- GST-Tag
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别名
- GST Tag
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物质类
- Tag
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背景
- Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the NSJBio anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.
抗原
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