This recombinant phospho-Histone H4 antibody reacts to Histone H4 phosphorylated at Serine 1. The reactivity is not affected by neighbor Arginine 3 modification. No cross reactivity with Histone H2A phosphorylated at Serine 1.
纯化方法
Protein A purified from animal origin-free supernatant
免疫原
A phospho-peptide corresponding to phospho-Histone H4 (pSer1) was used as the immunogen for this recombinant phospho-Histone H4 antibody.
适用: 人, 小鼠, 大鼠
WB, IF
宿主: 兔
Polyclonal
unconjugated
应用备注
The stated application concentrations are suggested starting points. Titration of the recombinant phospho-Histone H4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western Blot: 0.5-2 μg/mL,Immunocytochemistry: 1-2 μg/mL,ELISA: 0.2-1 μg/mL
限制
仅限研究用
缓冲液
1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Store the recombinant phospho-Histone H4 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
抗原
Histone H4
背景
This recombinant phospho-Histone H4 antibody reacts to Histone H4 phosphorylated at Serine 1. The reactivity is not affected by neighbor Arginine 3 modification. No cross reactivity with Histone H2A phosphorylated at Serine 1.