我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

Vimentin 抗体 (AA 152-181)

This 兔多克隆 antibody specifically detects Vimentin in WB, IHC, ELISA 和 FACS. It exhibits reactivity toward 人.

IHC analysis of FFPE human breast carcinoma tissue stained with VIM antibody (Vimentin 抗体 (AA 152-181))

IHC

VIM antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). (Vimentin 抗体 (AA 152-181))

FACS

Western blot analysis of VIM antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (2). (Vimentin 抗体 (AA 152-181))

VIM antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue. (Vimentin 抗体 (AA 152-181))

IHC

FACS

8 images

产品编号 ABIN3029593

发货至: 中国

Contact our Customer Service for availability and price in your country. Contact Info

Our Local Distributor

中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Vimentin 抗体 (AA 152-181) (ABIN3029593)

抗原

See all Vimentin (VIM) 抗体

Vimentin (VIM)

适用

人

宿主

兔

克隆类型

多克隆

标记

This Vimentin antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Discover our top product VIM Primary Antibody

抗原表位
40

32

28

16

14

12

12

11

8

7

5

5

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 152-181

原理

Vimentin Antibody

预测反应

Mouse,Rat,Bovine,Pig,Hamster

纯化方法

Purified

免疫原

A portion of amino acids 152-181 from the human protein was used as the immunogen for this VIM Antibody for FACS.

亚型

Ig Fraction
anti-Vimentin (VIM) (AA 371-466) antibody
KO Validated VIM 适用: 人 WB, FACS, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) (C-Term) antibody
VIM 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM 适用: 人 WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, 小鼠 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 哺乳动物 WB, FACS, IHC (p), ICC 宿主: 小鼠 Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, 大鼠, 小鼠, Pig, 小鸡 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, Pig, Cow, 小鸡, 犬, Cat, 山羊 WB, IHC, FACS 宿主: 小鼠 Monoclonal VM452 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, Pig, Cow, 小鸡, 犬, Cat, 马 WB, IHC, IF, FACS 宿主: 兔 Monoclonal VIM-1937R unconjugated Recombinant Antibody

anti-Vimentin (VIM) (AA 325-375) antibody
VIM 适用: 人, 小鼠 WB, IHC, ICC, IF (p) 宿主: 兔 Monoclonal BLR100G unconjugated Recombinant Antibody

应用备注

The stated application concentrations are suggested starting amounts. Titration of the VIM Antibody for FACS may be required due to differences in protocols and secondary/substrate sensitivity.

限制

仅限研究用
状态

Liquid

缓冲液

In 1X PBS, pH 7.4, with 0.09 % sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles

储存条件

-20 °C

储存方法

For long term storage, aliquot the VIM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
抗原

Vimentin (VIM)

别名

VIM

背景

Vimentin (VIM) is a type III intermediate filament protein that forms a cytoplasmic structural network in mesenchymal cells, where it regulates cell shape, migration, and cytoskeletal organization. VIM Antibody for FACS is specifically designed for flow cytometry applications, enabling intracellular detection of Vimentin at the single-cell level following fixation and permeabilization. This makes it highly relevant for searches such as Vimentin flow cytometry antibody, VIM FACS antibody, and intracellular Vimentin antibody for flow cytometry. Vimentin is also referred to as Vimentin antibody, VIM antibody, and mesenchymal marker antibody in the literature, supporting strong alignment across gene, protein, and functional terminology.

Flow cytometry analysis of Vimentin requires intracellular staining conditions, as Vimentin is not a surface protein but a cytoplasmic filament. VIM Antibody for FACS enables researchers to quantify Vimentin expression across heterogeneous cell populations, making it particularly useful for identifying mesenchymal cells, profiling epithelial-mesenchymal transition, and distinguishing stromal versus epithelial compartments in mixed samples. This application is especially valuable in cancer research, where Vimentin-positive populations are often associated with invasive, migratory, or therapy-resistant phenotypes.

At the functional level, Vimentin interacts with actin filaments and microtubules to regulate cellular mechanics, adhesion, and motility. Its dynamic reorganization through phosphorylation supports processes such as mitosis, migration, and cellular stress adaptation. These properties make Vimentin a key intracellular marker for flow cytometry studies focused on cell state transitions, immune activation, and cytoskeletal remodeling, particularly when combined with surface markers for multiparametric analysis.

The 'for FACS' differentiator is central to this antibody, clearly positioning it for flow cytometry workflows rather than tissue-based assays. A rabbit polyclonal VIM Antibody for FACS provides strong signal detection across permeabilized cells and supports population-level quantification of Vimentin / VIM expression. This makes it an effective tool for researchers performing intracellular flow cytometry, gating mesenchymal cell populations, and analyzing dynamic changes in cytoskeletal protein expression across diverse biological systems.

UniProt

P08670

途径

Caspase Cascade in Apoptosis