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Vimentin 抗体 (AA 152-181)

Name: Vimentin 抗体 (AA 152-181)
SKU: ABIN3029593
Availability: OutOfStock

VIM 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

Western blot analysis of VIM antibody and A2058, A375 lysate. (Vimentin 抗体 (AA 152-181))

IHC analysis of FFPE human breast carcinoma tissue stained with VIM antibody (Vimentin 抗体 (AA 152-181))

IHC

VIM antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram). (Vimentin 抗体 (AA 152-181))

FACS

Western blot analysis of VIM antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (2). (Vimentin 抗体 (AA 152-181))

VIM antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue. (Vimentin 抗体 (AA 152-181))

IHC

FACS

8 images

产品编号 ABIN3029593

发货至: 中国

Datasheet as PDF

抗原 See all Vimentin (VIM) 抗体

Vimentin (VIM)
抗原表位
32

18

16

16

16

14

11

8

8

7

4

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 152-181
适用
332

126

125

61

58

43

42

36

29

21

20

13

13

8

7

6

6

4

3

2

1

1

1

1

1

1

1
人
宿主
189

129

9

6

4

4

2
兔
Compatible Secondaries
克隆类型
197

145
多克隆
标记
199

19

13

13

11

7

7

7

7

7

7

7

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
应用范围
281

131

99

97

95

65

49

40

39

28

23

20

8

5

4

4

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

交叉反应（详细）

Expected species reactivity: Bovine, Hamster, Mouse, Pig, Rat

纯化方法

Purified

免疫原

A portion of amino acids 152-181 from the human protein was used as the immunogen for this VIM antibody.

亚型

Ig Fraction

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VIM 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5D7 unconjugated

应用备注

The stated application concentrations are suggested starting amounts. Titration of the VIM antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50

限制

仅限研究用
状态

Liquid

缓冲液

In 1X PBS pH 7.4 with 0.09 % sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

For long term storage, aliquot the VIM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
抗原

Vimentin (VIM)

别名

VIM (VIM 产品)

别名

CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody

背景

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. It is involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]

UniProt

P08670

途径

Caspase Cascade in Apoptosis