The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Growth Hormone antibody to be titered up or down for optimal performance.\. FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT
限制
仅限研究用
浓度
0.2 mg/mL
缓冲液
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Store the Growth Hormone antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
抗原
Pituitary Growth Hormone 20kDa
别名
Growth Hormone (pituitary)
背景
Pituitary growth hormone (GH) plays a crucial role in stimulating and controlling the growth, metabolism and differentiation of many mammalian cell types by modulating the synthesis of multiple mRNA species. These effects are mediated by the binding of GH to its membrane-bound receptor, GHR, and involve a phosphorylation cascade that results in the modulation of numerous signaling pathways. GH is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Anti-GH is a useful marker in classification of pituitary tumors and the study of pituitary disease (acromegaly).