Desmocollin 2+3 (Extracellular Domain) 抗体
适用: 人, 小鼠, 大鼠
IF, WB, FACS, IHC (p)
宿主: 小鼠
Monoclonal
7G6
unconjugated
1 image
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- 抗原
- Desmocollin 2+3
- 抗原表位
- Extracellular Domain
- 适用
- 人, 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- A recombinant protein corresponding to the extracellular domain of human Desmocollin 2 was used as the immunogen for the Desmocollin 2/3 antibody.
- 克隆位点
- 7G6
- 亚型
- IgG1 kappa
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- 应用备注
- Optimal dilution of the Desmocollin 2/3 antibody should be determined by the researcher.\. WB: 0.5-1 μg/mL,FACS: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Desmocollin 2/3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- Desmocollin 2+3
- 别名
- Desmocollin 2/3
- 背景
- Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. The desmosome is subdivided into two regions. The plaque region lies adjacent to the plasma, and is believed to contain molecules that attach the intermediate filament cytoskeleton to the desmosome. The core region is composed of transmembrane glycoproteins that are thought to mediate cell-cell adhesion. Desmogleins and desmocollins are the main desmosomal transmembrane proteins. These desmosomal glycoproteins belong to the members of the cadherin family of adhesion molecules. Three different isoforms of both desmogleins and desmocollins have been identified, named as desmoglein 1-3 and desmocollins. Desmosomal cadherins showed differentiation-specific expression in the human epidermis, although the functional significance of this differential expression is not fully understood. Desmocollin-1 can be found in the upper layers. The expression of Desmocollin-2 varies in the basal and suprabasal layers. And Desmocollin-3 is expressed more evenly throughout the suprabasal layers.
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