Recombinant CD8 alpha 抗体
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北京 101111
Quick Overview for Recombinant CD8 alpha 抗体 (ABIN4949538)
抗原
See all CD8 alpha (CD8A) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant full-length human CD8a protein was used as immunogen for this recombinant CD8 antibody.
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亚型
- IgG kappa
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应用备注
- The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CD8 antibody to be titered up or down for optimal performance.\. Flow Cytometry: 0.5-1 μg/million cells,Immunofluorescence: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the recombinant CD8 antibody (with azide) at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- CD8 alpha (CD8A) (CD8a Molecule (CD8A))
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别名
- CD8 (alpha chain)
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背景
- CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.
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途径
- TCR Signaling
抗原
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