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Recombinant S100B 抗体

This anti-S100B antibody is a 兔 单克隆 antibody detecting S100B in WB, IF, FACS 和 IHC (p). Suitable for 人, 大鼠, 小鼠 和 Cow.
产品编号 ABIN4949395
发货至: 中国

Quick Overview for Recombinant S100B 抗体 (ABIN4949395)

抗原

See all S100B 抗体
S100B (S100 Calcium Binding Protein B (S100B))

抗体类型

Recombinant Antibody

适用

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人, 大鼠, 小鼠, Cow

宿主

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克隆类型

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单克隆

标记

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This S100B antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

S100B-1706R
  • 纯化方法

    Protein A affinity chromatography

    免疫原

    Full length human recombinant S100B protein was used as the immunogen for this recombinant S100B antibody.

    亚型

    IgG kappa
  • 应用备注

    The optimal dilution of the recombinant S100B antibody for each application should be determined by the researcher.

    1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the recombinant S100B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    S100B (S100 Calcium Binding Protein B (S100B))

    别名

    S100B

    背景

    S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.

    途径

    Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins
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