Recombinant S100B 抗体
Quick Overview for Recombinant S100B 抗体 (ABIN4949393)
抗原
See all S100B 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein A affinity chromatography
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免疫原
- Full length human recombinant S100B protein was used as the immunogen for this recombinant S100B antibody.
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亚型
- IgG kappa
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anti-S100 Calcium Binding Protein B (S100B) (AA 31-92) antibody
S100B 适用: 大鼠 ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein B (S100B) (AA 1-92) antibodyS100B 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein B (S100B) antibodyS100B 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-S100 Calcium Binding Protein B (S100B) (AA 1-92) antibodyS100B 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein B (S100B) (AA 1-92) antibodyS100B 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein B (S100B) antibodyS100B 适用: 人, 大鼠, 小鼠, Cow WB, IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal 4C4-9 unconjugated
anti-S100 Calcium Binding Protein B (S100B) (AA 1-92) antibodyS100B 适用: 人 IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 4A5H7 unconjugated
anti-S100 Calcium Binding Protein B (S100B) antibodyS100B 适用: 人, 大鼠, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB22934 unconjugated
anti-S100 Calcium Binding Protein B (S100B) (AA 1-92) antibodyS100B 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein B (S100B) antibodyS100B 适用: 人, 大鼠, 小鼠, Cow IHC, StM 宿主: 兔 Monoclonal S100B-1706R unconjugated Recombinant Antibody
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应用备注
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The optimal dilution of the recombinant S100B antibody for each application should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
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限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the recombinant S100B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- S100B (S100 Calcium Binding Protein B (S100B))
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别名
- S100B
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背景
- S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.
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途径
- Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins
抗原
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