Recombinant PTH 抗体 (N-Term)
Quick Overview for Recombinant PTH 抗体 (N-Term) (ABIN4949384)
抗原
See all PTH 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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纯化方法
- Protein A affinity chromatography
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免疫原
- A synthetic peptide from the N-terminal region of human Parathyroid hormone was used as the immunogen for the recombinant PTH antibody.
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亚型
- IgG kappa
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应用备注
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Optimal dilution of the recombinant PTH antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the recombinant PTH antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- PTH (Parathyroid Hormone (PTH))
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别名
- Parathyroid Hormone
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物质类
- Hormone
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背景
- PTH/Parathyroid hormone is a hormone produced by the parathyroid gland that regulates the concentration of calcium and phosphorus in extracellular fluid. This hormone elevates blood Ca2+ levels by dissolving the salts in bone and preventing their renal excretion. It is produced in the parathyroid gland as an 84 amino acid single chain polypeptide. It can also be secreted as N-terminal truncated fragments or C-terminal fragments after intracellular degradation, as in case of hypercalcemia. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH), also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.
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途径
- cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process
抗原
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