Recombinant Chromogranin A 抗体
Quick Overview for Recombinant Chromogranin A 抗体 (ABIN4949322)
抗原
See all Chromogranin A (CHGA) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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无交叉反应
- 大鼠
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纯化方法
- Protein A affinity chromatography
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免疫原
- Recombinant human protein was used as the immunogen for the recombinant Chromogranin A antibody.
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亚型
- IgG kappa
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应用备注
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Optimal dilution of the recombinant Chromogranin A antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the recombinant Chromogranin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Chromogranin A (CHGA)
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别名
- Chromogranin A
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背景
- Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
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途径
- Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling
抗原
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