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Iba1 抗体 (C-Term)

This anti-Iba1 antibody is a 山羊 多克隆 antibody detecting Iba1 in WB, IHC (p) 和 EIA. Suitable for 人, 大鼠 和 小鼠.
产品编号 ABIN492698
发货至: 中国

Quick Overview for Iba1 抗体 (C-Term) (ABIN492698)

抗原

See all Iba1 (IBA1) 抗体
Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

适用

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人, 大鼠, 小鼠

宿主

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山羊

克隆类型

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多克隆

标记

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This Iba1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • 抗原表位

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    C-Term

    特异性

    This antibody recognizes Allograft Inflammatory Factor-1 (AIF1).

    交叉反应 (详细)

    Species reactivity (expected):Mouse, Bovine, Goat, Hamster, Rabbit, Pig, Horse, MonkeySpecies reactivity (tested):Human and Rat

    纯化方法

    Immunoaffinity Chromatography.

    免疫原

    Synthetic peptide (C-TGPPAKKAISELP) from C-terminus of Human AIF1

    亚型

    IgG
  • 应用备注

    ELISA: 1/128000. Western blot: 1-3 μg/mL. Immunohistochemistry on Paraffin Sections: 5 μg/mL. This antibody was validated for use in Immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by AlkalinePhosphatase-streptavidin and chromogen.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    Tris saline buffer, pH 7.3 containing 0.5 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Store the antibody in aliquots at-20 °C.
  • 抗原

    Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

    别名

    AIF1

    背景

    Allograft Inflammatory Factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with chronic inflammatory processes. AIF1 is expressed by activated monocytes and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts.Synonyms: AIF-1, Allograft inflammatory factor 1, IBA1, Ionized Calcium-Binding Adapter Molecule 1, Protein G1

    基因ID

    199

    NCBI登录号

    NP_001614

    UniProt

    P55008

    途径

    Smooth Muscle Cell Migration
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