Streptavidin 抗体
Quick Overview for Streptavidin 抗体 (ABIN492627)
抗原
See all Streptavidin 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- Synonyms: StAv
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纯化方法
- Subsequent Ultrafiltration and Size Exclusion Chromatography.
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免疫原
- Streptavidin
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亚型
- IgG1
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anti-Streptavidin (AA 25-183) antibody
适用: Streptomyces avidinii ELISA, WB 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Streptavidin (C-Term) antibody适用: Streptomyces avidinii WB 宿主: 山羊 Polyclonal unconjugated
anti-Streptavidin (AA 25-183) antibody适用: Streptomyces avidinii ELISA, WB 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Streptavidin antibody (FITC)适用: Streptomyces avidinii FACS, IF 宿主: 兔 Polyclonal FITC
anti-Streptavidin antibody适用: Streptomyces avidinii ELISA, WB, IHC, DB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: Use at 0.05 μg/mLImmunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Immunoprecipitation: Use at 0.05-1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1 mL H2O (15 min, RT).
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缓冲液
- 1 mLPBS, containing 0.09 % Sodium Azide/PEG and Sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
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有效期
- 12 months
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- Streptavidin
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背景
- Streptavidin is a tetrameric protein that binds tightly to a small growth factor Biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the Vitamin Biotin, the dissociation constant (Kd) of the Biotin-Streptavidin complex is on the order of ~10-15 Mol/L. The high affinity recognition of Biotin and biotinylated molecules has made Streptavidin one of the most important components in diagnostics and laboratory kits.Synonyms: StAv
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UniProt
- P22629
抗原
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