PTK6 抗体
Quick Overview for PTK6 抗体 (ABIN492612)
抗原
See all PTK6 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes BRK/PTK6
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产品特性
- Synonyms: Tyrosine-protein kinase 6, Breast tumor kinase, Tyrosine-protein kinase BRK
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纯化方法
- Subsequent Ultrafiltration and Size Exclusion Chromatography.
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免疫原
- Peptide conjugated to hemocyanin. Epitope: Internal peptide derived from BRK/PTK6 sequence
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亚型
- IgG1
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anti-PTK6 Protein tyrosine Kinase 6 (PTK6) (AA 1-451) antibody
PTK6 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-PTK6 Protein tyrosine Kinase 6 (PTK6) (AA 222-451) antibodyPTK6 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-PTK6 Protein tyrosine Kinase 6 (PTK6) (AA 191-445) antibodyPTK6 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-PTK6 Protein tyrosine Kinase 6 (PTK6) (AA 1-113) antibodyPTK6 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-PTK6 Protein tyrosine Kinase 6 (PTK6) (AA 342-451) antibodyPTK6 适用: 人 ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2F11 unconjugated
anti-PTK6 Protein tyrosine Kinase 6 (PTK6) antibodyPTK6 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H12B8 unconjugated
anti-PTK6 Protein tyrosine Kinase 6 (PTK6) (AA 11-41), (N-Term) antibodyPTK6 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB3073-3074 unconjugated
anti-PTK6 Protein tyrosine Kinase 6 (PTK6) (AA 342-452) antibodyPTK6 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2F11 unconjugated
anti-PTK6 Protein tyrosine Kinase 6 (PTK6) (AA 287-336) antibodyPTK6 适用: 人, 马 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Included Potitive Control: Cell lysate from untreated MDA-MB 468 cells (See Protocols formore details).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Positive Control: Cell lysate from untreated MDA-MB-468 cells, breast adenocarcinoma(Human)Format: Lyophilized cell lysate from serum starved MDA-MB-468 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended forapplications with native proteins such as in immunoprecipitation.
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限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- PBS containing 0.09 % Sodium Azide, PEG, Sucrose and 50 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
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Store the antibody in aliquots) at -20 °C. Avoid repeated freeze-thaw cycles.
Shelf life: one year from despatch. -
有效期
- 12 months
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- PTK6 (PTK6 Protein tyrosine Kinase 6 (PTK6))
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别名
- PTK6 / BRK
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背景
- This protein encoded is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. Its presence in the nucleus appears to be linked to suppression of tumor progression. The encoded protein has been shown to undergo autophosphorylation.Synonyms: Breast tumor kinase, Tyrosine-protein kinase 6, Tyrosine-protein kinase BRK
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基因ID
- 5753
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UniProt
- Q13882
抗原
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