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Aurora A 抗体 (C-Term)

Name: Aurora A 抗体 (C-Term)
SKU: ABIN492609
Availability: OutOfStock

This 小鼠单克隆 anti-Aurora A antibody (Clone 5F11) specifically detects Aurora A in WB, IF 和 FACS. The antibody is reactive with 人 samples.

Western Blotting (WB) image for anti-Aurora Kinase A (AURKA) (C-Term) antibody (ABIN492609) (Aurora A 抗体 (C-Term))

1 image

产品编号 ABIN492609

发货至: 中国

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Quick Overview for Aurora A 抗体 (C-Term) (ABIN492609)

抗原

See all Aurora A (AURKA) 抗体

Aurora A (AURKA) (Aurora Kinase A (AURKA))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This Aurora A antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

克隆位点

5F11

Discover our top product AURKA Primary Antibody

抗原表位
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6

3

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2

2

2

1

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1
C-Term

特异性

Recognizes Aurora-A/B (C-term).

产品特性

Synonyms: Aurora kinase A, Aurora A, Aurora B, AURKA, AURKB, AURA, AURB, BTAK, STK6, ARK1, STK15,AIK, AIK2, AIM1, ARK2, STK12

纯化方法

Size Exclusion Chromatography.

组件

incl. positive Control

免疫原

Peptide conjugated to hemocyaninEpitope: C-terminus.

亚型

IgG1
anti-Aurora Kinase A (AURKA) (AA 1-110) antibody
AURKA 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 5F8 unconjugated

anti-Aurora Kinase A (AURKA) (C-Term) antibody
AURKA 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Aurora Kinase A (AURKA) (AA 268-404) antibody
AURKA 适用: 人 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 2D8A4 unconjugated

anti-Aurora Kinase A (AURKA) (AA 364-392), (C-Term) antibody
AURKA 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB6834 unconjugated

anti-Aurora Kinase A (AURKA) antibody
AURKA 适用: 人, 大鼠, 猴 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1F8 unconjugated

anti-Aurora Kinase A (AURKA) (pThr288) antibody
AURKA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Aurora Kinase A (AURKA) antibody
AURKA 适用: 人, 大鼠, 猴 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 4G10 unconjugated

anti-Aurora Kinase A (AURKA) (AA 268-404) antibody
AURKA 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 4H5A8 unconjugated

anti-Aurora Kinase A (AURKA) (AA 364-392) antibody
AURKA 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-Aurora Kinase A (AURKA) (AA 1-100) antibody
AURKA 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

应用备注

Immunobotting (Western Blot): 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking buffer and blotincubation buffer. Included Postitive Control: Cell lysate from untreated SW620 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

实验流程

Positive Control: Cell lysate from untreated SW620 cells. Formulation: Lyophilized Cell lysate from Serum starved SW620 cells. Stability: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The Positive Control lysate is recommended for Immunoblot applications. 20µl of PositiveControl correspond to ca. 20. 000 cells. Use 20µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Shelf life: one year from despatch. The Lyophilized cell lysates contain SDS and are not recommended for applications withnative proteins such as Immunoprecipitation.

限制

仅限研究用
溶解方式

Restore with 1 mL H2O (15 min, RT).

缓冲液

PBS / 0.09 % Sodium Azide / PEG and Sucrose.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -20 °C to -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Shelf life: one year from despatch.

有效期

12 months
抗原

Aurora A (AURKA) (Aurora Kinase A (AURKA))

别名

Aurora Kinase A

背景

Aurora proteins are members of a serine/threonine kinase family. They play a crucial role in mitosis by regulating chromosome segregation and cytokinesis. There are three forms of Aurora proteins in mammalian cells: AuroraA, B and C. Aurora-A (Aurora-2, STK6, ARK1, Aurora/IPL-1 related kinase) associates with centrosomes and microtubules during mitosis. Phosphorylation of a threonine residue within the activation loop of the catalytic domain lead to activation of Aurora-A. AuroraB (Aurora-1) is responsible for chromatin modification and histone H3 phosphporylation.Synonyms: AIK, AIK2, AIM1, ARK1, ARK2, AURA, AURB, AURKA, AURKB, Aurora A, Aurora B, Aurora kinase A, BTAK, STK12, STK15, STK6

基因ID

6790

UniProt

O14965

途径

Cell Division Cycle, Asymmetric Protein Localization