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- 抗原 See all Aurora A (AURKA) 抗体
- Aurora A (AURKA) (Aurora Kinase A (AURKA))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Aurora A antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)
- 特异性
- Recognizes Aurora-A/B (C-term).
- 产品特性
- Synonyms: Aurora kinase A, Aurora A, Aurora B, AURKA, AURKB, AURA, AURB, BTAK, STK6, ARK1, STK15,AIK, AIK2, AIM1, ARK2, STK12
- 纯化方法
- Size Exclusion Chromatography.
- 组件
- incl. positive Control
- 免疫原
- Peptide conjugated to hemocyaninEpitope: C-terminus.
- 克隆位点
- 5F11
- 亚型
- IgG1
- Top Product
- Discover our top product AURKA Primary Antibody
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anti-Aurora Kinase A (AURKA) (AA 1-110) antibody
AURKA 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 5F8 unconjugated
anti-Aurora Kinase A (AURKA) (C-Term) antibodyAURKA 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Aurora Kinase A (AURKA) (AA 268-404) antibodyAURKA 适用: 人 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 2D8A4 unconjugated
anti-Aurora Kinase A (AURKA) (AA 364-392), (C-Term) antibodyAURKA 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB6834 unconjugated
anti-Aurora Kinase A (AURKA) antibodyAURKA 适用: 人 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1F8 unconjugated
anti-Aurora Kinase A (AURKA) (pThr288) antibodyAURKA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Aurora Kinase A (AURKA) antibodyAURKA 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 4G10 unconjugated
anti-Aurora Kinase A (AURKA) (AA 268-404) antibodyAURKA 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 4H5A8 unconjugated
anti-Aurora Kinase A (AURKA) (AA 364-392) antibodyAURKA 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Aurora Kinase A (AURKA) (AA 1-131) antibodyAURKA 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunobotting (Western Blot): 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking buffer and blotincubation buffer. Included Postitive Control: Cell lysate from untreated SW620 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Positive Control: Cell lysate from untreated SW620 cells. Formulation: Lyophilized Cell lysate from Serum starved SW620 cells. Stability: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The Positive Control lysate is recommended for Immunoblot applications. 20µl of PositiveControl correspond to ca. 20. 000 cells. Use 20µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Shelf life: one year from despatch. The Lyophilized cell lysates contain SDS and are not recommended for applications withnative proteins such as Immunoprecipitation.
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 1 mL H2O (15 min, RT).
- 缓冲液
- PBS / 0.09 % Sodium Azide / PEG and Sucrose.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -20 °C to -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- Aurora A (AURKA) (Aurora Kinase A (AURKA))
- 别名
- Aurora Kinase A (AURKA 产品)
- 背景
- Aurora proteins are members of a serine/threonine kinase family. They play a crucial role in mitosis by regulating chromosome segregation and cytokinesis. There are three forms of Aurora proteins in mammalian cells: AuroraA, B and C. Aurora-A (Aurora-2, STK6, ARK1, Aurora/IPL-1 related kinase) associates with centrosomes and microtubules during mitosis. Phosphorylation of a threonine residue within the activation loop of the catalytic domain lead to activation of Aurora-A. AuroraB (Aurora-1) is responsible for chromatin modification and histone H3 phosphporylation.Synonyms: AIK, AIK2, AIM1, ARK1, ARK2, AURA, AURB, AURKA, AURKB, Aurora A, Aurora B, Aurora kinase A, BTAK, STK12, STK15, STK6
- 基因ID
- 6790
- UniProt
- O14965
- 途径
- Cell Division Cycle, Asymmetric Protein Localization
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