Syntaxin 6 抗体
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- 抗原 See all Syntaxin 6 (STX6) 抗体
- Syntaxin 6 (STX6)
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适用
- 小鼠, 大鼠, Hamster
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Syntaxin 6 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
- 特异性
- This antibody reacts with 31 kDa membrane protein Syntaxin-6 on Western blotting and Immunoprecipitation. It is reported that this monoclonal antibody (3D10) binds to the amino-terminal 25 amino acid of Rat Syntaxin-6 (Ref.6). Detects a band of approximately 35 kDa (predicted molecular weight: 30.6 kDa).
- 交叉反应 （详细）
- Species reactivity (tested):Mouse, Hamster and Rat.It is reported that this clone 3D10 reacted with Human Syntaxin-6 in Reference 3.
- 产品特性
- Synonyms: Syntaxin-6, Golgi Marker
- 纯化方法
- Protein-A Sepharose Chromatography of hybridoma supernatant.
- 免疫原
- Recombinant Rat Syntaxin-6. Remarks: Hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/cmouse splenocyte
- 克隆位点
- 3D10
- 亚型
- IgG1
- Top Product
- Discover our top product STX6 Primary Antibody
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anti-Syntaxin 6 (STX6) (AA 1-255) antibody
STX6 适用: 人 ELISA, WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1G2 unconjugated
anti-Syntaxin 6 (STX6) antibodySTX6 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 6 (STX6) (Internal Region) antibodySTX6 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 6 (STX6) (Internal Region) antibodyVerified STX6 适用: 人 ELISA, WB 宿主: 山羊 Polyclonal unconjugated
anti-Syntaxin 6 (STX6) (AA 1-235) antibodySTX6 适用: 人, 小鼠, 大鼠, 小鸡, Hamster WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 6 (STX6) (AA 4-231) antibodySTX6 适用: 小鸡 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 6 (STX6) (AA 5-234) antibodySTX6 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Syntaxin 6 (STX6) (N-Term) antibodySTX6 适用: 人, 小鼠, 大鼠, 小鸡, Hamster, 犬, 马, 猴, Pig, Cow, Cat, 驴, 山羊, 豚鼠, 兔, 绵羊, Avian WB 宿主: 山羊 Polyclonal unconjugated
anti-Syntaxin 6 (STX6) antibodySTX6 适用: 人, 小鼠, 大鼠, Hamster WB, IP 宿主: 小鼠 Monoclonal 3D10 unconjugated
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- 应用备注
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Western blotting: 1 μg/mL for chemiluminescence detection system. Immunoprecipitation: 2 μg/300 μL of cell extract. Immunocytochemistry. Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- SDS-PAGE & Western Blotting1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10%glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. Measure the protein concentration of the supernatant and add the cold Lysisbuffer to make 8 mg/mL solution. 3) Mix the sample with equal volume of Laemmli’s sample buffer. 4) Boil the samples for 3 minutes and centrifuge. Load 10 μL of the sample per lane in a 1mm thick SDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm2 for 1 hourin a semi-dry transfer system (Transfer Buffer: 25 mM Tris, 190 mM glycine, 20% MeOH). See the manufacture's manual for precise transfer procedure. 6) To reduce nonspecific binding, soak the membrane in 10% skimmed milk (in PBS, pH7. 2) for 1 hour at room temperature, or overnight at 4°C. 7) Incubate the membrane with primary antibody diluted with PBS, pH 7. 2 containing 1%skimmed milk as suggest in the APPLICATIONS for 1 hour at room temperature. (Theconcentration of antibody will depend on condition. )8) Wash the membrane with PBS-T [0. 05% Tween-20 in PBS] (5 minutes x 3 times). 9) Incubate the membrane with the 1: 10,000 HRP-conjugated anti-mouse IgG diluted with1% skimmed milk (in PBS, pH 7. 2) for 1 hour at room temperature. 10) Wash the membrane with PBS-T (10 minutes x 3 times). 11) Wipe excess buffer on the membrane, then incubate it with appropriatechemiluminescence reagent for 1 minute. 12) Remove extra reagent from the membrane by dabbing with paper towel, and seal it inplastic wrap. 13) Expose to an X-ray film in a dark room for 3 minutes. 14) Develop the film as usual. The condition for exposure and development may vary. Positive Controls: Rat brain, WR19LImmunoprecipitation1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.2 containing 50 % Glycerol without preservatives.
- 储存液
- Without preservative
- 储存条件
- -20 °C
- 储存方法
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Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- Syntaxin 6 (STX6)
- 别名
- Syntaxin 6 / STX6 (STX6 产品)
- 别名
- CG7736 antibody, CT23545 antibody, Dm Syx6 antibody, DmSyx6 antibody, Dmel\\CG7736 antibody, SYN6 antibody, SYX6/10 antibody, Syx antibody, MGC78971 antibody, MGC132090 antibody, STX6 antibody, fb51b08 antibody, wu:fb51b08 antibody, zgc:110658 antibody, si:dkey-121a11.2 antibody, LOC734102 antibody, Syx6 antibody, Bmsyx6 antibody, Syntaxin-6 antibody, 2310039E05Rik antibody, 2410005I16Rik antibody, AA437559 antibody, AI415714 antibody, AI845861 antibody, Syntaxin 6 antibody, syntaxin 6 L homeolog antibody, syntaxin 6 antibody, Syx6 antibody, stx6.L antibody, STX6 antibody, stx6 antibody, Stx6 antibody
- 背景
- Key requirements for protein transport are vesicular carriers with a full complement of machinery to enable them to find and fuse with the correct downstream compartment. This machinery includes the soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein (SNAP) receptors (SNAREs). SNAREs mediate diverse membrane fusion events such as neurotransmitter-filled vesicles fusing with the presynaptic plasma membrane. Syntaxin-6 is a q-SNARE found in endosomal transport vesicles. Syntaxin-6 has been shown by electron microscopy to localize mostly to the trans-Golgi network (TGN) and, to a lesser extent, to the Golgi stack.Synonyms: Golgi Marker, Syntaxin-6
- 基因ID
- 58244
- UniProt
- Q9JKK1
- 途径
- Synaptic Vesicle Exocytosis
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