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CD3 抗体

CD3 适用: 人, 小鼠 FACS, IP, IHC, IHC (fro), WB, ICC 宿主: 小鼠 Monoclonal APA1-1 unconjugated
产品编号 ABIN490154
发货至: 中国
  • 抗原 See all CD3 抗体
    CD3
    适用
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    人, 小鼠
    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This CD3 antibody is un-conjugated
    应用范围
    • 274
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    Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunocytochemistry (ICC)
    特异性
    The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.
    纯化方法
    Protein A purified
    免疫原
    Purified human CD3 proteins isolated from thymus.

    Type of Immunogen: Purified protein
    克隆位点
    APA1-1
    亚型
    IgG1
    Top Product
    Discover our top product CD3 Primary Antibody
  • 应用备注
    Approved: Flo, ICC, IHC, IHC-Fr, IP, WB

    Usage: Flow Cytometry: Positive control - Human T cells stimulated with anti-CD3 (MEM-57) antibody (1 μg/mL). Sample preparation:. At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1 % BSA for 20 min. on ice. Wash
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS, 15 mM sodium azide,  pH 7.4
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    avoid freeze thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • 抗原
    CD3
    别名
    CD3 (CD3 产品)
    途径
    TCR Signaling, Ubiquitin Proteasome Pathway
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