H2AFZ 抗体 (C-Term)
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北京 101111
Quick Overview for H2AFZ 抗体 (C-Term) (ABIN4889649)
抗原
See all H2AFZ 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Histone H2A.Z antibody (pAb) (H2AZ)
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纯化方法
- Unpurified
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免疫原
- This Histone H2A.Z antibody was raised against a peptide derived from the C-terminus of human histone H2A.Z.
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anti-H2A Histone Family, Member Z (H2AFZ) (C-Term) antibody
H2AFZ 适用: 人 WB, ICC, IF, ChIP, ChIP-seq, DB 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member Z (H2AFZ) (H2A.ZK4ac) antibodyH2AFZ 适用: 人 WB, ELISA, ICC, IF, ChIP, IP 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member Z (H2AFZ) (H2A.ZK7ac) antibodyH2AFZ 适用: 人 WB, ELISA, ICC, IF, ChIP, IP 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member Z (H2AFZ) (C-Term) antibodyH2AFZ 适用: 人 WB, ICC, IF, ChIP, IP, IHC (p), IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member Z (H2AFZ) (N-Term) antibodyH2AFZ 适用: 人 WB, ICC, IF, IHC (p), IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member Z (H2AFZ) antibodyH2AFZ 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member Z (H2AFZ) (AA 59-86), (C-Term) antibodyH2AFZ 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB20139 unconjugated
anti-H2A Histone Family, Member Z (H2AFZ) (H2A.ZK4me1) antibodyH2AFZ 适用: 人 ELISA, ICC, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member Z (H2AFZ) antibodyH2AFZ 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Monoclonal 7G8 unconjugated
anti-H2A Histone Family, Member Z (H2AFZ) (AA 2-128) antibodyH2AFZ 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- ChIP: 2 - 10 µL per ChIP ChIP-Seq: 5 µL each WB*: 1:1,000 - 1:5,000 dilution This histone variant can be perturbed by excessive washing steps prior to fixation. Cells should be washed into serum-free culture medium (NOT PBS!) prior to fixation. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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: "Expression of Non-acetylatable H2A.Z in Myoblast Cells Blocks Myoblast Differentiation through Disruption of MyoD Expression." in: The Journal of biological chemistry, Vol. 290, Issue 21, pp. 13234-49, (2015) (PubMed).
: "Inactive or moderately active human promoters are enriched for inter-individual epialleles." in: Genome biology, Vol. 14, Issue 5, pp. R43, (2014) (PubMed).
: "CLOCK:BMAL1 is a pioneer-like transcription factor." in: Genes & development, Vol. 28, Issue 1, pp. 8-13, (2014) (PubMed).
: "The histone variant composition of centromeres is controlled by the pericentric heterochromatin state during the cell cycle." in: Journal of cell science, Vol. 127, Issue Pt 15, pp. 3347-59, (2014) (PubMed).
: "Elucidating combinatorial histone modifications and crosstalks by coupling histone-modifying enzyme with biotin ligase activity." in: Nucleic acids research, Vol. 41, Issue 3, pp. e49, (2013) (PubMed).
: "Coupling transcription factor occupancy to nucleosome architecture with DNase-FLASH." in: Nature methods, Vol. 11, Issue 1, pp. 66-72, (2013) (PubMed).
: "Microplate-based platform for combined chromatin and DNA methylation immunoprecipitation assays." in: BMC molecular biology, Vol. 12, pp. 49, (2011) (PubMed).
: "Dynamic and selective nucleosome repositioning during endotoxin tolerance." in: The Journal of biological chemistry, Vol. 285, Issue 2, pp. 1259-71, (2010) (PubMed).
: "Changes in H2A.Z occupancy and DNA methylation during B-cell lymphomagenesis." in: Genome research, Vol. 20, Issue 10, pp. 1383-90, (2010) (PubMed).
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- H2AFZ (H2A Histone Family, Member Z (H2AFZ))
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别名
- Histone H2A.Z
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背景
- The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle. H2A.Z is acetylated at multiple lysine residues in its amino terminus, which may enable H2A.Z to function as an insulator of chromatin domains.
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分子量
- 14 kDa
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基因ID
- 3015
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NCBI登录号
- NP_002097
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途径
- Telomere Maintenance
抗原
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