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VASP 抗体 (N-Term)

This anti-VASP antibody is a 兔 多克隆 antibody detecting VASP in WB 和 IHC. Suitable for 人 和 大鼠.
产品编号 ABIN4886759
发货至: 中国

Quick Overview for VASP 抗体 (N-Term) (ABIN4886759)

抗原

See all VASP 抗体
VASP (Vasodilator-Stimulated phosphoprotein (VASP))

适用

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人, 大鼠

宿主

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克隆类型

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多克隆

标记

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This VASP antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)
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    AA 78-114, N-Term

    原理

    Anti-VASP Antibody Picoband®

    序列

    NFHQWRDARQ VWGLNFGSKE DAAQFAAGMA SALEALE

    交叉反应 (详细)

    No cross-reactivity with other proteins

    产品特性

    Anti-VASP Antibody Picoband® (ABIN4886759). Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    A synthetic peptide corresponding to a sequence at the N-terminus of human VASP, different from the related mouse sequence by four amino acids.

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL, Human
    Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Rat
    1. Zimmer M, Fink T, Fischer L, Hauser W, Scherer K, Lichter P, Walter U (January 1997). "Cloning of the VASP (vasodilator-stimulated phosphoprotein) genes in human and mouse: structure, sequence, and chromosomal localization". Genomics. 36 (2): 227-33. 2. Harbeck, B, Hüttelmaier S, Schluter K, Jockusch B M, Illenberger S (October 2000). "Phosphorylation of the vasodilator-stimulated phosphoprotein regulates its interaction with actin". J. Biol. Chem. UNITED STATES.275 (40): 30817-25. 3. Reinhard, M, Giehl K, Abel K, Haffner C, Jarchau T, Hoppe V, Jockusch B M, Walter U (April 1995). "The proline-rich focal adhesion and microfilament protein VASP is a ligand for profilins". EMBO J. ENGLAND.14 (8): 1583-9.

    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原

    VASP (Vasodilator-Stimulated phosphoprotein (VASP))

    别名

    VASP

    背景

    Synonyms: Vasodilator-stimulated phosphoprotein,VASP,VASP,

    Tissue Specificity: Highly expressed in platelets. .

    Background: Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

    分子量

    40 kDa

    基因ID

    7408

    UniProt

    P50552

    途径

    TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation
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