TPSAB1 抗体 (AA 65-275)
Quick Overview for TPSAB1 抗体 (AA 65-275) (ABIN4886754)
抗原
See all TPSAB1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 65-275
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原理
- Anti-Mast Cell Tryptase/TPSAB1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Mast Cell Tryptase/TPSAB1 Antibody Picoband® (ABIN4886754). Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human Mast Cell Tryptase recombinant protein (Position: H65-P275). Human Mast Cell Tryptase shares 77% and 76.1% amino acid (aa) sequence identity with mouse and rat Mast Cell Tryptase, respectively.
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亚型
- IgG
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anti-Tryptase alpha/beta 1 (TPSAB1) (AA 161-262) antibody
TPSAB1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tryptase alpha/beta 1 (TPSAB1) (AA 161-262) antibodyTPSAB1 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Tryptase alpha/beta 1 (TPSAB1) (AA 161-262) antibodyTPSAB1 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C17 unconjugated
anti-Tryptase alpha/beta 1 (TPSAB1) (AA 151-275) antibodyTPSAB1 适用: 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tryptase alpha/beta 1 (TPSAB1) (AA 1-275) antibodyTPSAB1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2A10-B5 unconjugated
anti-Tryptase alpha/beta 1 (TPSAB1) (AA 19-210) antibodyTPSAB1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Tryptase alpha/beta 1 (TPSAB1) (AA 115-233) antibodyTPSAB1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal TPSAB1-1961 unconjugated
anti-Tryptase alpha/beta 1 (TPSAB1) (AA 149-273) antibodyTPSAB1 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Tryptase alpha/beta 1 (TPSAB1) (AA 151-275) antibodyTPSAB1 适用: 犬 WB 宿主: 小鼠 Monoclonal C13 unconjugated
anti-Tryptase alpha/beta 1 (TPSAB1) (AA 1-275) antibodyTPSAB1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
ELISA(Cap) , 1-5 μg/mL, Human, -
1. "Entrez Gene: TPSAB1 tryptase alpha/beta 1". 2. Miller JS, Moxley G, Schwartz LB (Oct 1990). "Cloning and characterization of a second complementary DNA for human tryptase". J Clin Invest 86 (3): 864-70. 3. Pallaoro M, Fejzo MS, Shayesteh L, Blount JL, Caughey GH (Feb 1999). "Characterization of genes encoding known and novel human mast cell tryptases on chromosome 16p13.3". J Biol Chem 274 (6): 3355-62. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))
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别名
- TPSAB1
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背景
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Synonyms: Tryptase alpha/beta-1,Tryptase-1,3.4.21.59,Tryptase I,Tryptase alpha-1,TPSAB1,TPS1, TPS2, TPSB1,
Tissue Specificity: Isoform 1 and isoform 2 are expressed in lung, stomach, spleen, heart and skin, in these tissues, isoform 1 is predominant. Isoform 2 is expressed in aorta, spleen, and breast tumor, with highest levels in the endothelial cells of some blood vessels surrounding the aorta, as well as those surrounding the tumor and low levels, if any, in mast cells (at protein level). .
Background: Tryptase alpha-1 and tryptase beta-1 are enzymes that in humans are encoded by the same TPSAB1 gene. Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. In addition, these genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.
Sequence Similarities: Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.
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分子量
- 30 kDa
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基因ID
- 7177
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UniProt
- Q15661
抗原
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