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LDHB 抗体 (AA 237-334)

LDHB 适用: 人, 大鼠, 小鼠, 猴 WB, IHC, ICC, IF, FACS 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN4886658
发货至: 中国
  • 抗原 See all LDHB 抗体
    LDHB (Lactate Dehydrogenase B (LDHB))
    抗原表位
    • 20
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 237-334
    适用
    • 35
    • 22
    • 21
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    人, 大鼠, 小鼠, 猴
    宿主
    • 40
    • 8
    • 2
    克隆类型
    • 40
    • 9
    多克隆
    标记
    • 30
    • 7
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This LDHB antibody is un-conjugated
    应用范围
    • 39
    • 19
    • 15
    • 12
    • 12
    • 12
    • 7
    • 6
    • 5
    • 5
    • 4
    • 4
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
    原理
    Anti-Lactate Dehydrogenase B/LDHB Antibody Picoband®
    交叉反应 (详细)
    No cross-reactivity with other proteins
    产品特性
    Anti-Lactate Dehydrogenase B/LDHB Antibody Picoband® (ABIN4886658). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    纯化方法
    Immunogen affinity purified.
    免疫原
    E. coli-derived human LDHB recombinant protein (Position: E237-L334). Human LDHB shares 98% amino acid (aa) sequence identity with both mouse and rat LDHB.
    亚型
    IgG
    Top Product
    Discover our top product LDHB Primary Antibody
  • 应用备注
    Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Markert, C. L. Lactate dehydrogenase: biochemistry and function of lactate dehydrogenase. Cell Biochem. Func. 2: 131-134, 1984. 2. Okumura, N., Terasawa, F., Ueno, I., Oki, K., Yamauchi, K., Hidaka, H., Tozuka, M., Okura, M., Katsuyama, T. Genetic analyses in homozygous and heterozygous variants of lactate dehydrogenase-B (H) subunit: LD-B Matsumoto I and II (LD-B W323R). Clin. Chim. Acta 287: 163-171, 1999.
    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C,-20 °C
    储存方法
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原
    LDHB (Lactate Dehydrogenase B (LDHB))
    别名
    LDHB (LDHB 产品)
    背景

    Synonyms: L-lactate dehydrogenase B chain,LDH-B,1.1.1.27,LDH heart subunit,LDH-H,Renal carcinoma antigen NY-REN-46,LDHB,

    Tissue Specificity: Low levels in brain. Very weak expression found in heart and muscle. .

    Background: The LDHB gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13.

    分子量
    37 kDa
    基因ID
    3945
    UniProt
    P07195
    途径
    Warburg Effect
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