LDHB 抗体 (AA 237-334)
Quick Overview for LDHB 抗体 (AA 237-334) (ABIN4886658)
抗原
See all LDHB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 237-334
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原理
- Anti-Lactate Dehydrogenase B/LDHB Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Lactate Dehydrogenase B/LDHB Antibody Picoband® (ABIN4886658). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human LDHB recombinant protein (Position: E237-L334). Human LDHB shares 98% amino acid (aa) sequence identity with both mouse and rat LDHB.
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亚型
- IgG
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anti-Lactate Dehydrogenase B (LDHB) (AA 1-334) antibody
LDHB 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase B (LDHB) (AA 1-334) antibodyLDHB 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase B (LDHB) (AA 1-334) antibodyLDHB 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase B (LDHB) (AA 1-334) antibodyLDHB 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2H6 unconjugated
anti-Lactate Dehydrogenase B (LDHB) antibodyKO Validated LDHB 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase B (LDHB) (C-Term) antibodyLDHB 适用: 人, 大鼠, 小鼠, Cow, 马, 犬, 兔, 豚鼠, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase B (LDHB) (C-Term) antibodyLDHB 适用: 人, 猴, 犬, 兔, 小鸡 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase B (LDHB) (AA 1-335) antibodyLDHB 适用: 人 WB, IHC, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2H6 unconjugated
anti-Lactate Dehydrogenase B (LDHB) (N-Term) antibodyLDHB 适用: 人, 大鼠, 小鼠 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lactate Dehydrogenase B (LDHB) (AA 220-250) antibodyLDHB 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB47345 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Markert, C. L. Lactate dehydrogenase: biochemistry and function of lactate dehydrogenase. Cell Biochem. Func. 2: 131-134, 1984. 2. Okumura, N., Terasawa, F., Ueno, I., Oki, K., Yamauchi, K., Hidaka, H., Tozuka, M., Okura, M., Katsuyama, T. Genetic analyses in homozygous and heterozygous variants of lactate dehydrogenase-B (H) subunit: LD-B Matsumoto I and II (LD-B W323R). Clin. Chim. Acta 287: 163-171, 1999. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- LDHB (Lactate Dehydrogenase B (LDHB))
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别名
- LDHB
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背景
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Synonyms: L-lactate dehydrogenase B chain,LDH-B,1.1.1.27,LDH heart subunit,LDH-H,Renal carcinoma antigen NY-REN-46,LDHB,
Tissue Specificity: Low levels in brain. Very weak expression found in heart and muscle. .
Background: The LDHB gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13.
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分子量
- 37 kDa
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基因ID
- 3945
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UniProt
- P07195
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途径
- Warburg Effect
抗原
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