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LDHB 抗体 (AA 237-334)

This anti-LDHB antibody is a 兔 多克隆 antibody detecting LDHB in WB, IHC, ICC, IF 和 FACS. Suitable for 人, 大鼠, 小鼠 和 猴.
产品编号 ABIN4886658
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for LDHB 抗体 (AA 237-334) (ABIN4886658)

抗原

See all LDHB 抗体
LDHB (Lactate Dehydrogenase B (LDHB))

适用

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人, 大鼠, 小鼠, 猴

宿主

  • 61
  • 16
  • 2

克隆类型

  • 50
  • 28
多克隆

标记

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This LDHB antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
  • 抗原表位

    • 28
    • 4
    • 1
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    AA 237-334

    原理

    Anti-Lactate Dehydrogenase B/LDHB Antibody

    交叉反应 (详细)

    No cross-reactivity with other proteins

    产品特性

    Anti-Lactate Dehydrogenase B/LDHB Antibody (ABIN4886658). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

    纯化方法

    Immunogen affinity purified.

    免疫原

    E. coli-derived human LDHB recombinant protein (Position: E237-L334). Human LDHB shares 98% amino acid (aa) sequence identity with both mouse and rat LDHB.

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human

    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原

    LDHB (Lactate Dehydrogenase B (LDHB))

    别名

    LDHB

    背景

    Synonyms: L-lactate dehydrogenase B chain,LDH-B,1.1.1.27,LDH heart subunit,LDH-H,Renal carcinoma antigen NY-REN-46,LDHB,

    Tissue Specificity: Low levels in brain. Very weak expression found in heart and muscle. .

    Background: The LDHB gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13.

    分子量

    37 kDa

    基因ID

    3945

    UniProt

    P07195

    途径

    Warburg Effect
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