HNRNPF 抗体 (N-Term)
Quick Overview for HNRNPF 抗体 (N-Term) (ABIN4886622)
抗原
See all HNRNPF 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 23-53, N-Term
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原理
- Anti-HnRNP F/HNRNPF Antibody Picoband®
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序列
- SVEDVQNFLS DCTIHDGAAG VHFIYTREGR Q
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-HnRNP F/HNRNPF Antibody Picoband® (ABIN4886622). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human HnRNP F, different from the related mouse and rat sequences by two amino acids.
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亚型
- IgG
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anti-Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF) antibody
KO Validated HNRNPF 适用: 人 WB, IF, ICC, IHC (p), IP 宿主: 兔 Polyclonal unconjugated
anti-Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF) (C-Term) antibodyHNRNPF 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 兔, 犬, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF) antibodyHNRNPF 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3H4 unconjugated
anti-Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF) (N-Term) antibodyHNRNPF 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF) (N-Term) antibodyHNRNPF 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF) (N-Term) antibodyHNRNPF 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF) (C-Term) antibodyHNRNPF 适用: 人, 小鼠, 大鼠, 豚鼠, 兔, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF) (AA 1-280) antibodyHNRNPF 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF) (C-Term) antibodyHNRNPF 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF) antibodyHNRNPF 适用: 人 WB, IF, IHC, FACS 宿主: 小鼠 Monoclonal 5F5 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. "Entrez Gene: HNRPF heterogeneous nuclear ribonucleoprotein F". 2. Honore B, Rasmussen HH, Vorum H, Dejgaard K, Liu X, Gromov P, Madsen P, Gesser B, Tommerup N, Celis JE (Jan 1996). "Heterogeneous nuclear ribonucleoproteins H, H', and F are members of a ubiquitously expressed subfamily of related but distinct proteins encoded by genes mapping to different chromosomes". J Biol Chem. 270 (48): 28780-9. 3. Matunis MJ, Xing J, Dreyfuss G (1994). "The hnRNP F protein: unique primary structure, nucleic acid-binding properties, and subcellular localization.". Nucleic Acids Res. 22 (6): 1059-67. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))
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别名
- HNRNPF
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背景
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Synonyms: Heterogeneous nuclear ribonucleoprotein F,hnRNP F,Nucleolin-like protein mcs94-1,Heterogeneous nuclear ribonucleoprotein F, N-terminally processed,HNRNPF,HNRPF,
Tissue Specificity: Expressed ubiquitously.
Background: Heterogeneous nuclear ribonucleoprotein F is a protein that in humans is encoded by the HNRNPF gene. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.
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分子量
- 46 kDa
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基因ID
- 3185
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UniProt
- P52597
抗原
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