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EpCAM 抗体 (Middle Region)

There are 2+ publications for this product available. The 兔 多克隆 anti-EpCAM antibody is suitable to detect EpCAM in samples from 人. It has been validated for WB, FACS, IHC, IF 和 ELISA.
产品编号 ABIN4886570
发货至: 中国
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北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for EpCAM 抗体 (Middle Region) (ABIN4886570)

抗原

See all EpCAM (EPCAM) 抗体
EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

适用

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宿主

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克隆类型

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多克隆

标记

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This EpCAM antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA
  • 抗原表位

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    Middle Region

    原理

    Anti-EpCAM Antibody

    序列

    ELKHKAREKP YDSKSLRTAL QKEITTRYQL DPKFITSILY ENN

    特异性

    No cross reactivity with other proteins.

    交叉反应 (详细)

    No cross-reactivity with other proteins

    预测反应

    different from the related mouse sequence by fifteen amino acids,and from the related rat sequence by sixteen amino acids.

    产品特性

    Anti-EpCAM Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human.

    纯化方法

    Immunogen affinity purified.

    免疫原

    A synthetic peptide corresponding to a sequence in the middle region of human EPCAM, different from the related mouse sequence by fifteen amino acids, and from the related rat sequence by sixteen amino acids.

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL Immunofluorescence, 5 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x106cells ELISA, 0.1-0.5 μg/mL

    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

    有效期

    12 months
  • Huang, Li, Pan, Cheng, Ren, Jia, Ma, Xu: "A novel multi-target RNAi adenovirus inhibits hepatoma cell proliferation, migration, and induction of angiogenesis." in: Oncotarget, Vol. 7, Issue 36, pp. 57705-57713, (2018) (PubMed).

    Zhang, Bu, Sun, Xu, Yao, He, Lai: "Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 270, (2018) (PubMed).

  • 抗原

    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

    别名

    EPCAM

    背景

    Background: Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.

    Gene Full Name: epithelial cell adhesion molecule

    分子量

    35-40 kDa

    基因ID

    4072

    UniProt

    P16422
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