EpCAM 抗体 (Middle Region)
(2 references)Quick Overview for EpCAM 抗体 (Middle Region) (ABIN4886570)
抗原
See all EpCAM (EPCAM) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 147-189, Middle Region
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原理
- Anti-EpCAM Antibody Picoband®
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序列
- ELKHKAREKP YDSKSLRTAL QKEITTRYQL DPKFITSILY ENN
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-EpCAM Antibody Picoband® (ABIN4886570). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human EPCAM, different from the related mouse sequence by fifteen amino acids, and from the related rat sequence by sixteen amino acids.
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亚型
- IgG
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anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 40-260) antibody
EPCAM 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 53-150) antibodyEPCAM 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 25-265) antibodyEPCAM 适用: Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 20-60), (N-Term) antibodyEPCAM 适用: 人 FACS, IHC, StM 宿主: 兔 Monoclonal EGP40-1555R unconjugated Recombinant Antibody
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibodyEPCAM 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal rEGP40-1372 unconjugated Recombinant Antibody
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibodyEPCAM 适用: 人 FACS, IF, IHC, ELISA, StM, Coat 宿主: 小鼠 Monoclonal EGP40-1384 unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 280-350), (Cytoplasmic Domain) antibodyEPCAM 适用: 人, 小鼠, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal EGP40-1110 unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibodyEPCAM 适用: 人 WB, FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal EGP40-1373 unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibodyEPCAM 适用: 人 WB, FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal EGP40-1372 unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) antibodyEPCAM 适用: 人 WB, FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 7B5C10 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL
Immunofluorescence, 5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106cells
ELISA, 0.1-0.5 μg/mL
1. Litvinov, Sergey, et al. (1994). "Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule". The Journal of Cell Biology 125 (2): 437-46. 2. Maetzel, Dorothea, et al. (2009). "Nuclear signalling by tumour-associated antigen EpCAM". Nature Cell Biology 11 (2): 162-71. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "A novel multi-target RNAi adenovirus inhibits hepatoma cell proliferation, migration, and induction of angiogenesis." in: Oncotarget, Vol. 7, Issue 36, pp. 57705-57713, (2018) (PubMed).
: "Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 270, (2018) (PubMed).
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: "A novel multi-target RNAi adenovirus inhibits hepatoma cell proliferation, migration, and induction of angiogenesis." in: Oncotarget, Vol. 7, Issue 36, pp. 57705-57713, (2018) (PubMed).
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- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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别名
- EPCAM
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背景
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Synonyms: Epithelial cell adhesion molecule,Ep-CAM,Adenocarcinoma-associated antigen,Cell surface glycoprotein Trop-1,Epithelial cell surface antigen,Epithelial glycoprotein,EGP,Epithelial glycoprotein 314,EGP314,hEGP314,KS 1/4 antigen,KSA,Major gastrointestinal tumor-associated protein GA733-2,Tumor-associated calcium signal transducer 1,CD326,EPCAM,GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1,
Tissue Specificity: Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .
Background: Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
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分子量
- 35-40 kDa
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基因ID
- 4072
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UniProt
- P16422
抗原
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