Emerin 抗体 (N-Term)
Quick Overview for Emerin 抗体 (N-Term) (ABIN4886569)
抗原
See all Emerin (EMD) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-48, N-Term
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原理
- Anti-Emerin/EMD Antibody Picoband®
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序列
- MDNYADLSDT ELTTLLRRYN IPHGPVVGST RRLYEKKIFE YETQRRRL
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Emerin/EMD Antibody Picoband® (ABIN4886569). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human Emerin, different from the related mouse sequence by eight amino acids, and from the related rat sequence by nine amino acids.
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亚型
- IgG
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anti-Emerin (EMD) (AA 56-167) antibody
EMD 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal EMD-2168 unconjugated
anti-Emerin (EMD) (AA 56-167) antibodyEMD 适用: 人 WB, IHC, IF, StM 宿主: 小鼠 Monoclonal EMD-2167 unconjugated
anti-Emerin (EMD) (AA 1-222) antibodyEMD 适用: 人 ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 8F5A8 unconjugated
anti-Emerin (EMD) (AA 1-222) antibodyEMD 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Emerin (EMD) (AA 1-110) antibodyEMD 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3B9 unconjugated
anti-Emerin (EMD) (AA 1-254) antibodyEMD 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Emerin (EMD) (AA 1-220) antibodyEMD 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Emerin (EMD) antibodyEMD 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Emerin (EMD) (AA 1-188) antibodyEMD 适用: 人, 兔 WB, IF, ICC 宿主: 小鼠 Monoclonal MANEM1(5D10) unconjugated
anti-Emerin (EMD) (AA 114-183) antibodyEMD 适用: 小鼠, 大鼠, Hamster WB, IF, ICC, EM 宿主: 绵羊 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunofluorescence, 2 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. "Entrez Gene: EMD emerin (Emery-Dreifuss muscular dystrophy)". 2. Lammerding, J, Hsiao, J, Schulze, PC, Kozlov, S, Stewart, CL, Lee, RT (29 August 2005). "Abnormal nuclear shape and impaired mechanotransduction in emerin-deficient cells.". The Journal of Cell Biology. 170 (5): 781-91. 3. Nagano A, Koga R, Ogawa M, Kurano Y, Kawada J, Okada R, Hayashi YK, Tsukahara T, Arahata K (Mar 1996). "Emerin deficiency at the nuclear membrane in patients with Emery-Dreifuss muscular dystrophy". Nature Genetics. 12 (3): 254-9. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Emerin (EMD)
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别名
- EMD
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背景
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Synonyms: Emerin,EMD,EDMD, STA,
Tissue Specificity: Skeletal muscle, heart, colon, testis, ovary and pancreas.
Background: Emerin is a serine-rich nuclear membrane protein that in humans is encoded by the EMD gene. And this gene is mapped to Xq28. Emerin is a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Emery-Dreifuss muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the EMD (also known clinically as STA) gene. Emerin appears to be involved in mechanotransduction, as emerin-deficient mouse fibroblasts failed to transduce normal mechanosensitive gene expression responses to strain stimuli. In cardiac muscle, emerin is also found complexed to beta-catenin at adherens junctions of intercalated discs, and cardiomyocytes from hearts lacking emerin showed beta-catenin redistribution as well as perturbed intercalated disc architecture and myocyte shape. This interaction appears to be regulated by glycogen synthase kinase 3 beta.
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分子量
- 34 kDa
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基因ID
- 2010
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UniProt
- P50402
抗原
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