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DDAH2 抗体 (C-Term)

This anti-DDAH2 antibody is a 兔 多克隆 antibody detecting DDAH2 in WB, IHC, IF, ICC 和 FACS. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN4886561
发货至: 中国

Quick Overview for DDAH2 抗体 (C-Term) (ABIN4886561)

抗原

See all DDAH2 抗体
DDAH2 (Dimethylarginine Dimethylaminohydrolase 2 (DDAH2))

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

  • 30
  • 13
多克隆

标记

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This DDAH2 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
  • 抗原表位

    • 10
    • 4
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    AA 190-224, C-Term

    原理

    Anti-DDAH2 Antibody Picoband®

    序列

    DAAQKAVRAM AVLTDHPYAS LTLPDDAAAD CLFLR

    交叉反应 (详细)

    No cross-reactivity with other proteins

    产品特性

    Anti-DDAH2 Antibody Picoband® (ABIN4886561). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    纯化方法

    Immunogen affinity purified.

    免疫原

    A synthetic peptide corresponding to a sequence at the C-terminus of human DDAH2, different from the related mouse and rat sequences by three amino acids.

    亚型

    IgG
  • 应用备注

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Leiper, J. M, Santa Maria, J., Chubb, A., MacAllister, R. J., Charles, I. G., Whitley, G. S., Vallance, P. Identification of two human dimethylarginine dimethylaminohydrolases with distinct tissue distributions and homology with microbial arginine deiminases.Biochem. J. 343: 209-214, 1999. 2. Smith, C. L., Birdsey, G. M., Anthony, S., Arrigoni, F. I., Leiper, J. M., Vallance, P. Dimethylarginine dimethylaminohydrolase activity modulates ADMA levels, VEGF expression, and cell phenotype. Biochem. Biophys. Res. Commun. 308: 984-989, 2003. 3. Tran, C. T. L., Fox, M. F., Vallance, P., Leiper, J. M. Chromosomal localization, gene structure, and expression pattern of DDAH1: comparison with DDAH2 and implications for evolutionary origins. Genomics 68: 101-105, 2000.

    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    浓度

    500 μg/mL

    缓冲液

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原

    DDAH2 (Dimethylarginine Dimethylaminohydrolase 2 (DDAH2))

    别名

    DDAH2

    背景

    Synonyms: N (G),N (G)-dimethylarginine dimethylaminohydrolase 2,DDAH-2,Dimethylarginine dimethylaminohydrolase 2,3.5.3.18,DDAHII,Dimethylargininase-2,Protein G6a,S-phase protein,DDAH2,DDAH, G6A, NG30,

    Tissue Specificity: Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain. .

    Background: DDAH2 is known as dimethylarginine dimethylaminohydrolase 2 which is mapped to 6p21.3 by radiation hybrid and FISH analysis. This gene encodes a dimethylarginine dimethylaminohydrolase. DDAH2 functions in nitric oxide generation by regulating the cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. The protein may be localized to the mitochondria. Alternative splicing resulting in multiple transcript variants.

    Sequence Similarities: Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

    分子量

    24 kDa

    基因ID

    23564

    UniProt

    O95865
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