CDCP1 抗体 (AA 582-667)
Quick Overview for CDCP1 抗体 (AA 582-667) (ABIN4886532)
抗原
See all CDCP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 582-667
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原理
- Anti-CDCP1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-CDCP1 Antibody Picoband® (ABIN4886532). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human CDCP1 recombinant protein (Position: R582-T667). Human CDCP1 shares 84.5% amino acid (aa) sequence identity with mouse CDCP1.
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亚型
- IgG
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anti-CUB Domain Containing Protein 1 (CDCP1) (AA 689-836) antibody
CDCP1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CUB Domain Containing Protein 1 (CDCP1) antibodyCDCP1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CUB Domain Containing Protein 1 (CDCP1) (Internal Region) antibodyCDCP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CUB Domain Containing Protein 1 (CDCP1) (AA 692-836) antibodyCDCP1 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 5G3H11 unconjugated
anti-CUB Domain Containing Protein 1 (CDCP1) antibodyCDCP1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-CUB Domain Containing Protein 1 (CDCP1) (N-Term) antibodyCDCP1 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CUB Domain Containing Protein 1 (CDCP1) antibodyCDCP1 适用: 人, 小鼠, 猴, 犬 WB, FACS 宿主: 小鼠 Monoclonal 5B3 unconjugated
anti-CUB Domain Containing Protein 1 (CDCP1) (Extracellular Domain) antibodyCDCP1 适用: 人, 猴, 马 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CUB Domain Containing Protein 1 (CDCP1) (C-Term) antibodyVerified CDCP1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-CUB Domain Containing Protein 1 (CDCP1) (AA 30-343) antibodyCDCP1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. "Entrez Gene: CDCP1 CUB domain containing protein 1". 2. Scherl-Mostageer M, Sommergruber W, Abseher R, Hauptmann R, Ambros P, Schweifer N (Jul 2001). "Identification of a novel gene, CDCP1, overexpressed in human colorectal cancer". Oncogene 20 (32): 4402-8. 3. Spassov DS, Ahuja D, Wong CH, Moasser MM (2011). "The structural features of trask that mediate its anti-adhesive functions". PLoS ONE 6 (4): e19154. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- CDCP1 (CUB Domain Containing Protein 1 (CDCP1))
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别名
- CDCP1
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背景
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Synonyms: CUB domain-containing protein 1,Membrane glycoprotein gp140,Subtractive immunization M plus HEp3-associated 135 kDa protein,SIMA135,Transmembrane and associated with src kinases,CD318,CDCP1,TRASK,UNQ2486/PRO5773,
Tissue Specificity: Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells. .
Background: CUB domain-containing protein 1 (CDCP1) is a protein that in humans is encoded by the CDCP1 gene. It has also been designated as CD318 (cluster of differentiation 318) and Trask (Transmembrane and associated with src kinases). CDCP1/Trask is a 140 kD transmembrane glycoprotein with a large extracellular domain (ECD) containing two CUB domains, and a smaller intracellular domain (ICD) containing five tyrosines. The tyrosine phosphorylation of Trask is tightly regulated and reciprocally linked with the state of cell adhesion. The tyrosine phosphorylation of CDCP1 in cultured cells occurs when cells are induced to detach by trypsin or EDTA, or seen spontaneously during mitotic detachment. The overexpression of CDCP1 leads to the loss of cell adhesion and a detached phenotype. CDCP1 is widely expressed in human epithelial tissues, but its phosphorylation is only seen in mitotically detached or shedding cells, consistent with its role in the negative regulation of cell adhesion.
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分子量
- 130 kDa
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基因ID
- 64866
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UniProt
- Q9H5V8
抗原
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