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CD58 抗体 (AA 29-215)

This anti-CD58 antibody is a 兔多克隆 antibody detecting CD58 in FACS, WB 和 IHC. Suitable for 人.

Western blot analysis of LFA3 expression in HELA whole cell lysates ( Lane 1) and K562 whole cell lysates ( Lane 2). (CD58 抗体 (AA 29-215))

Flow Cytometry analysis of Hela cells using anti-LFA3 antibody . (CD58 抗体 (AA 29-215))

LFA3 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- LFA3 Antigen Affinity purified polyclonal antibody (Catalog # ) at 1 µg/mL. (CD58 抗体 (AA 29-215))

3 images

产品编号 ABIN4886530

发货至: 中国

Quick Overview for CD58 抗体 (AA 29-215) (ABIN4886530)

抗原

See all CD58 抗体

CD58

适用

人

宿主

兔

克隆类型

多克隆

标记

41
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2
2
2
1
1
1
1
1
1
1
1
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This CD58 antibody is un-conjugated

应用范围

50
35
15
13
13
13
11
11
6
5
4
4
2

Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

Discover our top product CD58 Primary Antibody

抗原表位
15

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 29-215

原理

Anti-LFA3/CD58 Antibody Picoband®

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-LFA3/CD58 Antibody (ABIN4886530). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

E. coli-derived human LFA3 recombinant protein (Position: F29-R215).

亚型

IgG
anti-CD58 (CD58) (AA 1-240) antibody
CD58 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 2D11-B10 unconjugated

anti-CD58 (CD58) (AA 1-214) antibody
CD58 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD58 (CD58) (AA 31-130) antibody
CD58 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-CD58 (CD58) (AA 40-220) antibody
CD58 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-CD58 (CD58) (AA 1-241) antibody
CD58 适用: 人 WB, ELISA, IHC, IHC (p), IF 宿主: 小鼠 Monoclonal 2D11-B10 unconjugated

anti-CD58 (CD58) antibody
CD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 unconjugated

anti-CD58 (CD58) antibody (Biotin)
CD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 Biotin

anti-CD58 (CD58) antibody (APC)
CD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 APC

anti-CD58 (CD58) antibody (PE)
CD58 适用: 人, Pig FACS 宿主: 小鼠 Monoclonal MEM-63 PE

anti-CD58 (CD58) antibody (iFluor™488)
GMP Grade CD58 适用: 人 FACS 宿主: 小鼠 Monoclonal TS2-9 iFluor™488

应用备注

Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Flow Cytometry, 1-3 μg/1x10⁶cells
1. Barbosa, J. A., Mentzer, S. J., Kamarck, M. E., Hart, J., Biro, P. A., Strominger, J. L., Burakoff, S. J. Gene mapping and somatic cell hybrid analysis of the role of human lymphocyte function-associated antigen-3 (LFA-3) in CTL-target cell interactions. J. Immun. 136: 3085-3091, 1986. 2. Sewell, W. A., Palmer, R. W., Spurr, N. K., Sheer, D., Brown, M. H., Bell, Y., Crumpton, M. J. The human LFA-3 gene is located at the same chromosome band as the gene for its receptor CD2. Immunogenetics 28: 278-282, 1988. 3. Wang, J., Smolyar, A., Tan, K., Liu, J., Kim, M., Sun, Z. J., Wagner, G., Reinherz, E. L. Structure of a heterophilic adhesion complex between the human CD2 and CD58 (LFA-3) counterreceptors. Cell 97: 791-803, 1999.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
抗原

CD58

别名

CD58

背景

Synonyms: Lymphocyte function-associated antigen 3,Ag3,Surface glycoprotein LFA-3,CD58,CD58,LFA3,
Tissue Specificity: Widely expressed.
Background: CD58, or lymphocyte function-associated antigen 3 (LFA-3), is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. It binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. This adhesion occurs as part of the transitory initial encounters between T cells and Antigen Presenting Cells before T cell activation, when T cells are roaming the lymph nodes looking at the surface of APCs for peptide:MHC complexes the T-cell receptors are reactive to. The LFA3 gene is mapped to chromosome 1p13, which is the same location as CD2.

分子量

26 kDa

基因ID

965

UniProt

P19256

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