APOB 抗体 (AA 246-450)
Quick Overview for APOB 抗体 (AA 246-450) (ABIN4886467)
抗原
See all APOB 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 246-450
-
原理
- Anti-Apolipoprotein B/APOB Antibody Picoband®
-
交叉反应 (详细)
- No cross-reactivity with other proteins
-
产品特性
- Anti-Apolipoprotein B/APOB Antibody Picoband® (ABIN4886467). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- E. coli-derived human Apolipoprotein B recombinant protein (Position: Q246-N450). Human Apolipoprotein B shares 82.4% and 80.5% amino acid (aa) sequence identity with mouse and rat Apolipoprotein B, respectively.
-
亚型
- IgG
-
-
-
-
应用备注
-
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Jacobson TA (2011). "Opening a new lipid "apo-thecary": incorporating apolipoproteins as potential risk factors and treatment targets to reduce cardiovascular risk". Mayo Clinic Proceedings 86 (8): 762-780. 2. Lim JS, Lee DH, Park JY, Jin SH, Jacobs DR (2011). "Reliability of low-absorbance lipoprotein cholesterol, non-high-absorbance lipoprotein cholesterol, and apolipoprotein B measurement".Journal of Clinical Lipidology 5 (4): 264-272. -
说明
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
- APOB (Apolipoprotein B (APOB))
-
别名
- APOB
-
背景
-
Synonyms: Apolipoprotein B-100,Apo B-100,Apolipoprotein B-48,Apo B-48,APOB,
Tissue Specificity: Widely expressed (at protein level). Specifically expressed in the CNS. .
Background: Apolipoprotein B (ApoB) is a protein that in humans is encoded by the APOB gene. This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.
-
分子量
- 250 kDa
-
基因ID
- 338
-
UniProt
- P04114
-
途径
- Lipid Metabolism
抗原
-