AMFR 抗体 (AA 553-643)
Quick Overview for AMFR 抗体 (AA 553-643) (ABIN4886455)
抗原
See all AMFR 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 553-643
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原理
- Anti-AMFR Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-AMFR Antibody Picoband® (ABIN4886455). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human AMFR recombinant protein (Position: E553-S643). Human AMFR shares 89% amino acid (aa) sequence identity with mouse AMFR.
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亚型
- IgG
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anti-Autocrine Motility Factor Receptor (AMFR) (AA 456-643) antibody
AMFR 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Autocrine Motility Factor Receptor (AMFR) (AA 571-601) antibodyAMFR 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Autocrine Motility Factor Receptor (AMFR) (AA 571-601) antibodyAMFR 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB4403-4404 unconjugated
anti-Autocrine Motility Factor Receptor (AMFR) (AA 456-639) antibodyAMFR 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Autocrine Motility Factor Receptor (AMFR) (AA 485-643) antibodyAMFR 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Autocrine Motility Factor Receptor (AMFR) (C-Term) antibodyAMFR 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Autocrine Motility Factor Receptor (AMFR) antibodyAMFR 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Autocrine Motility Factor Receptor (AMFR) (AA 371-400) antibodyAMFR 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Autocrine Motility Factor Receptor (AMFR) (AA 451-550) antibodyAMFR 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3D9 unconjugated
anti-Autocrine Motility Factor Receptor (AMFR) (AA 110-159) antibodyAMFR 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, Bat, 小鸡, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Lee, Joon No, Zhang Xiangyu, Feramisco Jamison D, Gong Yi, Ye Jin (Nov 2008). "Unsaturated fatty acids inhibit proteasomal degradation of Insig-1 at a postubiquitination step". J. Biol. Chem. (United States)283 (48): 33772-83. 2. Watanabe H, Carmi P, Hogan V, Raz T, Silletti S, Nabi IR, Raz A (Aug 1991). "Purification of human tumor cell autocrine motility factor and molecular cloning of its receptor". J Biol Chem 266 (20): 13442-8. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- AMFR (Autocrine Motility Factor Receptor (AMFR))
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别名
- AMFR
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背景
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Synonyms: E3 ubiquitin-protein ligase AMFR,6.3.2.-,Autocrine motility factor receptor,AMF receptor,RING finger protein 45,gp78,AMFR,RNF45,
Tissue Specificity: Abundant in hepatoma cells and fetal cochlea, ovary, eye, heart, adrenal gland, liver and kidney. Low levels present in adult peripheral blood leukocytes and fetal brain, thymus, spleen, skeletal muscle, lung and tongue. .
Background: Autocrine motility factor receptor, isoform 2 is a protein that in humans is encoded by the AMFR gene. Autocrine motility factor is a tumor motility-stimulating protein secreted by tumor cells. The protein encoded by this gene is a glycosylated transmembrane protein and a receptor for autocrine motility factor. The receptor, which shows some sequence similarity to tumor protein p53, is localized to the leading and trailing edges of carcinoma cells. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins.
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分子量
- 73 kDa
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基因ID
- 267
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途径
- ER-Nucleus Signaling
抗原
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