Adenylate Kinase 1 抗体 (C-Term)
Quick Overview for Adenylate Kinase 1 抗体 (C-Term) (ABIN4886445)
抗原
See all Adenylate Kinase 1 (AK1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 149-189, C-Term
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原理
- Anti-Adenylate Kinase 1/AK1 Antibody Picoband®
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序列
- RLETYYKATE PVIAFYEKRG IVRKVNAEGS VDSVF
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-Adenylate Kinase 1/AK1 Antibody Picoband® (ABIN4886445). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human Adenylate Kinase 1, different from the related mouse sequence by seven amino acids, and from the related rat sequence by four amino acids.
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亚型
- IgG
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Bianchi, P., Zappa, M., Bredi, E., Vercellati, C., Pelissero, G., Barraco, F., Zanella, A. A case of complete adenylate kinase deficiency due to a nonsense mutation in AK-1 gene (arg107-to-stop, CGA-to-TGA) associated with chronic haemolytic anaemia. Brit. J. Haemat. 105: 75-79, 1999. 2. Qualtieri, A., Pedace, V., Bisconte, M. G., Bria, M., Gulino, B., Andreoli, V., Brancati, C. Severe erythrocyte adenylate kinase deficiency due to homozygous A-to-G substitution at codon 164 of human AK1 gene associated with chronic haemolytic anaemia. Brit. J. Haemat. 99: 770-776, 1997. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Adenylate Kinase 1 (AK1)
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别名
- AK1
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背景
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Synonyms: Adenylate kinase isoenzyme 1 ,AK 1 ,2.7.4.3 ,2.7.4.6 ,ATP-AMP transphosphorylase 1 ,ATP:AMP phosphotransferase ,Adenylate monophosphate kinase ,Myokinase ,AK1 ,
Tissue Specificity: Widely expressed. Highest level in placenta, liver, muscle and kidney.
Background: This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
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分子量
- 22 kDa
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基因ID
- 203
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UniProt
- P00568
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途径
- Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process
抗原
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