AFF4 抗体 (N-Term)
Quick Overview for AFF4 抗体 (N-Term) (ABIN4886436)
抗原
See all AFF4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 6-53, N-Term
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原理
- Anti-AFF4 Antibody Picoband®
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序列
- RNVLRMKERE RRNQEIQQGE DAFPPSSPLF AEPYKVTSKE DKLSSRIQ
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-AFF4 Antibody Picoband® (ABIN4886436). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human AFF4, identical to the related mouse sequence.
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亚型
- IgG
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anti-AF4/FMR2 Family, Member 4 (AFF4) (AA 1-365) antibody
AFF4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-AF4/FMR2 Family, Member 4 (AFF4) (AA 1-108) antibodyAFF4 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2E12 unconjugated
anti-AF4/FMR2 Family, Member 4 (AFF4) (AA 1-365) antibodyAFF4 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-AF4/FMR2 Family, Member 4 (AFF4) (C-Term) antibodyAFF4 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-AF4/FMR2 Family, Member 4 (AFF4) (AA 77-334) antibodyAFF4 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-AF4/FMR2 Family, Member 4 (AFF4) (AA 1-108) antibodyAFF4 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-AF4/FMR2 Family, Member 4 (AFF4) (AA 751-800) antibodyAFF4 适用: 人, 大鼠, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-AF4/FMR2 Family, Member 4 (AFF4) (AA 1-220) antibodyAFF4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-AF4/FMR2 Family, Member 4 (AFF4) (Middle Region) antibodyAFF4 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Izumi, K., Nakato, R., Zhang, Z., Edmondson, A. C., Noon, S., Dulik, M. C., Rajagopalan, R., Venditti, C. P., Gripp, K., Samanich, J., Zackai, E. H., Deardorff, M. A., and 10 others. Germline gain-of-function mutations in AFF4 cause a developmental syndrome functionally linking the super elongation complex and cohesin. Nature Genet. 47: 338-344, 2015. 2. Taki, T., Kano, H., Taniwaki, M., Sako, M., Yanagisawa, M., Hayashi, Y. AF5q31, a newly identified AF4-related gene, is fused to MLL in infant acute lymphoblastic leukemia with ins(5,11)(q31,q31q23). Proc. Nat. Acad. Sci. 96: 14535-14540, 1999. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- AFF4 (AF4/FMR2 Family, Member 4 (AFF4))
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别名
- AFF4
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背景
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Synonyms: AF4/FMR2 family member 4,ALL1-fused gene from chromosome 5q31 protein,Protein AF-5q31,Major CDK9 elongation factor-associated protein,AFF4,AF5Q31, MCEF,HSPC092,
Tissue Specificity: Ubiquitously expressed. Strongly expressed in heart, placenta, skeletal muscle, pancreas and to a lower extent in brain. .
Background: The AFF4 gene encodes a scaffold protein that functions as a core component of the super elongation complex (SEC), which is involved in transcriptional regulation during embryogenesis. The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. This gene is mapped to chromosome 5q31.
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分子量
- 150 kDa
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基因ID
- 27125
抗原
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