ADO 抗体 (AA 49-261)
Quick Overview for ADO 抗体 (AA 49-261) (ABIN4886431)
抗原
See all ADO 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 49-261
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原理
- Anti-ADO Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-ADO Antibody Picoband® (ABIN4886431). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human ADO recombinant protein (Position: E49-E261). Human ADO shares 90.1% amino acid (aa) sequence identity with mouse ADO.
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亚型
- IgG
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anti-2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO) antibody
ADO 适用: 人, 小鼠, 大鼠 WB, FACS 宿主: 兔 Polyclonal unconjugated
anti-2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO) antibodyADO 适用: 人, 小鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO) (AA 137-270) antibodyADO 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO) (AA 206-233), (C-Term) antibodyADO 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB30095 unconjugated
anti-2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO) (AA 206-233), (C-Term) antibodyADO 适用: 人, 小鼠 WB, EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Dominy, J. E., Jr., Simmons, C. R., Hirschberger, L. L., Hwang, J., Coloso, R. M., Stipanuk, M. H. Discovery and characterization of a second mammalian thiol dioxygenase, cysteamine dioxygenase.J. Biol. Chem. 282: 25189-25198, 2007. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))
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别名
- ADO
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背景
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Synonyms: 2-aminoethanethiol dioxygenase,1.13.11.19,Cysteamine dioxygenase,ADO,C10orf22,
Tissue Specificity: Widely expressed. Highest level in placenta, liver, muscle and kidney.
Background: Human thiol dioxygenases include cysteine dioxygenase (CDO) and cysteamine (2-aminoethanethiol) dioxygenase (ADO). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. It is demonstrated that mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. It is also noted that the demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.
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分子量
- 28 kDa, 30 kDa
抗原
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