Ricin 抗体
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北京 101111
Quick Overview for Ricin 抗体 (ABIN488640)
抗原
适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-Ricin (RTA subunit), clone MMA
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交叉反应 （详细）
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Ricinus communis
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Ricinus communis
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纯化方法
- Purified IgG
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免疫原
- Ricin
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亚型
- IgG1
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应用备注
- The optimal working dilution should be determined by the investigator, for specific application
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说明
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Detected epitope is located on the surface of RTA subunit not accssible in intact ricin molecule. This antibody does not cross-react to whole ricin molecule.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 200 μL of sterile water
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缓冲液
- Purified IgG in PBS pH 7.4 with mannitol.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- 4 °C,-80 °C
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储存方法
- Store at -70°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. , For storage at 4°C sodium azide can be added
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- Ricin
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背景
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Background: Ricin is a protein found in castor bean (Ricinus communis) acting as protein synthesis inhibitor. It is a toxin involved in plant defence. A chain of ricin can inactivate few thousands of ribosomes per minute. B chain facilitates the entry of A chain into the cell.
Additional information: Antibodies have been purified by affinity chromatography on Protein G from tissue culture supernatant and lyophilized from solution containing: mannitol 1 % , dextran 1 %, NaCl 100 mM, sodium phosphate 10 mM, pH 7.5. If required, mannitol can be removed either by dialysis or by chromatography using mini columns Sephadex G50 fine. The concentration can be measured by absorbance at 280 nm (1.35 for 1 mg/mL) or by Bradford methodRicin detection limit is 5 ng/mL, signal-to-noise ratio is 15-50 up to 100 ng/mLEpitope recognized by this antibody is located on the surface of RTA subunit and not accessible in intact ricin molecule. Suggested protocol can be found here.
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分子量
- 29.8 kDa
抗原
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