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AGO1 抗体 (N-Term)

Name: AGO1 抗体 (N-Term)
SKU: ABIN488536
Availability: OutOfStock

EIF2C1 适用: Arabidopsis thaliana, Nicotiana benthamiana WB 宿主: 兔 Polyclonal unconjugated

Image no. 1 for anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (N-Term) antibody (ABIN488536)

1 image

(1 reference)

产品编号 ABIN488536

发货至: 中国

Datasheet as PDF

抗原 See all AGO1 (EIF2C1) 抗体

AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))
抗原表位
9

4

2

2

2

1

1

1
N-Term
适用
32

17

7

3

3

2

2

2

2

2

1

1

1

1

1

1

1
Arabidopsis thaliana, Nicotiana benthamiana
宿主
34

1

1

1
兔
Compatible Secondaries
克隆类型
33

4
多克隆
标记
24

3

2

1

1

1

1

1

1

1

1
This AGO1 antibody is un-conjugated
应用范围
24

13

9

7

3

3

3

1

1

1

1
Western Blotting (WB)

特异性

Antibody binds microRNA and tasiRNAs, preference for 21ntmiRNAs with 5'U

交叉反应（详细）

Not reactive in: Chlamydomonas reinhardtii

预测反应

Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Solanum tuberosum, Zea mays, Vitis vinifera

产品特性

Expected / apparent Molecular Weight of the Antigene: 116.4 / 130 kDa
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA, immunoblotting and enzyme inhibition.

纯化方法

serum

免疫原

N-terminal peptide of Arabidopsis thaliana AGO1 O04379, At1g48410

Top Product

Discover our top product EIF2C1 Primary Antibody
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 376-409) antibody
EIF2C1 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) antibody
EIF2C1 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (Internal Region) antibody
EIF2C1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (C-Term) antibody
EIF2C1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 1-180) antibody
EIF2C1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 251-400) antibody
EIF2C1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (Internal Region) antibody
Verified EIF2C1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (N-Term) antibody
EIF2C1 适用: 人, 小鼠, 大鼠, 犬, 猴, Cow, 马, 兔, 豚鼠, Pig, 非洲爪蟾, Bat WB 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 27-56) antibody
EIF2C1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 27-56), (N-Term) antibody
EIF2C1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB18965 unconjugated

应用备注

1: 5000 - 1: 10 000 (WB)

说明

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

限制

仅限研究用
状态

Lyophilized

溶解方式

For reconstitution add 50 μL of sterile water.

注意事项

Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

储存条件

-20 °C

储存方法

store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Speth, Willing, Rausch, Schneeberger, Laubinger: "RACK1 scaffold proteins influence miRNA abundance in Arabidopsis." in: The Plant journal : for cell and molecular biology, Vol. 76, Issue 3, pp. 433-45, (2013) (PubMed).
抗原

AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

别名

AGO1 (EIF2C1 产品)

别名

Ago antibody, Ago-1 antibody, Ago1 antibody, CG6671 antibody, Dm Ago1 antibody, Dmel\\CG6671 antibody, MRE20 antibody, ago1 antibody, ago1-1 antibody, anon-WO0257455.29 antibody, dAGO1 antibody, dAgo1 antibody, l(2)04845 antibody, l(2)4845 antibody, l(2)k00208 antibody, l(2)k08121 antibody, GB12654 antibody, dsim_GLEANR_9718 antibody, DsimGD25729 antibody, GD25729 antibody, EIF2C1 antibody, EIF2C antibody, GERP95 antibody, Q99 antibody, Eif2c1 antibody, ARGONAUTE 1 antibody, T1N15.2 antibody, T1N15_2 antibody, Argonaute-1 antibody, protein argonaute-2 antibody, argonaute 1 antibody, Argonaute 1 antibody, protein argonaute-1 antibody, argonaute 1, RISC catalytic component antibody, argonaute RISC catalytic subunit 1 antibody, Stabilizer of iron transporter SufD / Polynucleotidyl transferase antibody, AGO1 antibody, LOC552062 antibody, ago1 antibody, LOC659936 antibody, Dsim\AGO1 antibody, Ago1 antibody, LOC100386910 antibody, LOC100482671 antibody, LOC475337 antibody

背景

AGI Code: At1g48410
AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

分子量

expected: 116.4 kDa, apparent: 130 kDa

UniProt

O04379

途径

Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization