AGO1 抗体 (N-Term)
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北京 101111
Quick Overview for AGO1 抗体 (N-Term) (ABIN488536)
抗原
See all AGO1 (EIF2C1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- Anti-AGO1 | Argonaute 1
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特异性
- Antibody binds microRNA and tasiRNAs, preference for 21ntmiRNAs with 5'U
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交叉反应 （详细）
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Arabidopsis thaliana, Hyacinthus orientalis, Nicotiana benthamiana
Not reactive in: Chlamydomonas reinhardtii, Medicago sativa, Salvia sp., Spinacia oleracea, Oryza sativa, Phaseolus vulgaris, Triticum aestivum, Zea maysMammals -
预测反应
- Brassica pekinensis,Capsella rubella,Cucumis sativus,Glycine max,Malus domestica,Pisum sativum,Ricinus communis,Solanum lycopersicum,Solanum tuberosum,Vitis vinifera
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纯化方法
- Immunogen affinity purified serum
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免疫原
- KLH-conjugated
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anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 1-180) antibody
EIF2C1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) antibodyEIF2C1 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (Internal Region) antibodyEIF2C1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 251-400) antibodyEIF2C1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (Internal Region) antibodyVerified EIF2C1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 27-56) antibodyEIF2C1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (N-Term) antibodyEIF2C1 适用: 人, 小鼠, 大鼠, 犬, 猴, 兔, 马, Cow, 豚鼠, Pig, 非洲爪蟾, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (N-Term) antibodyEIF2C1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 27-56), (N-Term) antibodyEIF2C1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB18965 unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (N-Term) antibodyEIF2C1 适用: 人, 小鼠, 大鼠, 犬, 兔, 马, Cow, 斑马鱼, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- 2 μg (ChIP), 1: 200 (IF), (IL), small-RNA-IP-Seq, 1 : 5000-1 : 10 000 (WB)
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说明
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AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Immunogen affinity purified serum in PBS pH 7.4.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "RACK1 scaffold proteins influence miRNA abundance in Arabidopsis." in: The Plant journal : for cell and molecular biology, Vol. 76, Issue 3, pp. 433-45, (2013) (PubMed).
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: "RACK1 scaffold proteins influence miRNA abundance in Arabidopsis." in: The Plant journal : for cell and molecular biology, Vol. 76, Issue 3, pp. 433-45, (2013) (PubMed).
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- AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))
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别名
- AGO1
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背景
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Background: AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
Additional information: Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U.To detect AGO1 in Nicotiana benthamiana, please inquire.Recommended for detection of AGO1: extreme low femtogram range chemiluminescent detection reagent
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分子量
- 116.4 | 130 kDa
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基因ID
- 841262
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UniProt
- O04379
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途径
- Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization
抗原
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