SELPLG 抗体 (FITC)
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北京 101111
Quick Overview for SELPLG 抗体 (FITC) (ABIN488144)
抗原
See all SELPLG 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody reacts with Human PSGL-1 antigen.
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Protein-A Sepharose Chromatography.
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免疫原
- Human Neutrophil PSGL-1. Remarks: This Hybridoma was established by fusion of mouse myeloma cell P3-X63-Ag8-653 with Balb/c mouse splenocyte.
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亚型
- IgG1
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anti-Selectin P Ligand (SELPLG) (AA 251-350) antibody
SELPLG 适用: 人 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Selectin P Ligand (SELPLG) antibody (PE)SELPLG 适用: 人 FACS 宿主: 小鼠 Monoclonal TC2 PE
anti-Selectin P Ligand (SELPLG) (AA 94-345) antibodySELPLG 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Selectin P Ligand (SELPLG) (N-Term) antibodySELPLG 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Selectin P Ligand (SELPLG) antibodySELPLG 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal PSGL1-1601 unconjugated
anti-Selectin P Ligand (SELPLG) antibody (FITC)SELPLG 适用: 人 FACS 宿主: 小鼠 Monoclonal TC2 FITC
anti-Selectin P Ligand (SELPLG) antibody (APC)SELPLG 适用: 人 FACS 宿主: 小鼠 Monoclonal TC2 APC
anti-Selectin P Ligand (SELPLG) (N-Term) antibodySELPLG 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Selectin P Ligand (SELPLG) antibodySELPLG 适用: 人 IHC, ELISA 宿主: 兔 Monoclonal 3G11 unconjugated Recombinant Antibody
anti-Selectin P Ligand (SELPLG) antibodySELPLG 适用: 人 FACS 宿主: 兔 Chimeric unconjugated Fc fragment
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应用备注
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Flow Cytometry: 10-20 μg/mL (final concentration). Positive Controls: Jurkat, HL60.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Flow Cytometric Analysis for Floating CellsProtocol 1We use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Add 10 µL of normal goat serum to the cell pellet aftertapping. Mix well, and incubate for 5 minutes at roomtemperature (20~25°C). 3) Add 30 µL of the FITC labeled CD162 (PL2) monoclonal antibody (10-20 µg/mL) dilutedwith the washing buffer. Mix well, and incubate for 30 minutes at room temperature(20~25°C). 4) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 5) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Protocol 2We usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with PBS containing 25% normal goat serum, 1 mg/mL normalhuman IgG and 0. 1% NaN3 (5x10e6 cells/mL). 3) Add 20 µL of the non diluted FITC labeled CD162 (PL1) monoclonal antibody (50 µg/mL)into each tube. 4) Add 50 µL of the cell suspension into each tube. Mix well, and incubate for 30 minutesat room temperature (20~25°C)5) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 6) Resuspend the cells with 500 μL of the washing buffer and analyze by a flow cytometer.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.05 mg/mL
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缓冲液
- PBS, pH 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store the antibody undiluted at 2-8 °C.
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- SELPLG (Selectin P Ligand (SELPLG))
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别名
- CD162 / PSGL1
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背景
- CD162 is a 120kD molecule also known as P-Selectin Glycoprotein Ligand I (PSGL-1). CD162 is the major counter receptor for P-selectin and is involved in neutrophil attachment and rolling to endothelium. In peripheral blood CD162 is expressed by monocytes, granulocytes, T cells and some B cells.Synonyms: P-selectin glycoprotein ligand 1, PSGL-1, SELPLG, Selectin P ligand
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基因ID
- 6404
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NCBI登录号
- NP_002997
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UniProt
- Q14242
抗原
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